[Histonet] MMA

[Myri37 <@t> aol.com]

hello everyone
am writting you about MMA embedding
i had a protocol using only MMA with peroxide benzoyle, embedding at 27 C°, tissue was preserved, but immunostainnig was impossible
and we found another protocol using MMA, methylbenzoate, PEG 400, butylmetacrylate, this one preserves tissue for immunostaining,and it's embedded at -20°C 

does anyone use the first or the other, wich one is better for light microscopy ?  ou do you have other protocols ?  
i would like to preserve mineralized tissue, and its interface with a metal biomaterial
any information or advice would be helpfull
Thank you 
Myriam