[Histonet] Re: Best fixative for Mouse lung [from Histonet Digest, Vol 3, Issue 25]

[Todd Sherman]

-----BEGIN PGP SIGNED MESSAGE-----
Hash: SHA1

Hello Laurie,

It's been a while since I've done the fixation but I would cast my vote
for 4% paraformaldehyde (fresh) in PBS buffer. Perfusion would be ideal,
but if the tissue is small enough, immersion works fine. We usually
immersed/fixed the tissue in cool fixative (i.e. refrigerated
temperatures) until it was time to process and paraffin infiltrate in an
automated processor. At that point, routine automated processing in 10%
NBF was the norm (if any buffered formalin steps were used at all).

Hope this helps,
Todd


Todd Sherman
President
HistoSoft Corporation
www.histosoft.com   - Home
www.myhistosoft.com - Member Services

histonet-request <@t> lists.utsouthwestern.edu wrote:

| When replying, please edit your Subject line so it is more specific
| than "Re: Contents of Histonet digest..."
|
|
| Today's Topics:
|
|   10. Best fixative for Mouse lung (Starkus, Laurie)
| ----------------------------------------------------------------------
Message: 10
Date: Thu, 19 Feb 2004 12:43:31 -0500
From: "Starkus, Laurie" <StarkusL <@t> ummhc.org>
Subject: [Histonet] Best fixative for Mouse lung
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<E9F72FA12FC6D411AD750006298F2F8205878844 <@t> hcunivexch04.ummhc.org>
Content-Type: text/plain;	charset="iso-8859-1"

For those of you doing rodent lung, what is your preferred fixative and I
assume perfusion is your best method?
- ------------------------------
-----BEGIN PGP SIGNATURE-----
Version: GnuPG v1.2.3 (MingW32)
Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org

iD8DBQFANQP7EmHXdrslGRcRArh1AJ4si0JM/AHO2VGih8c5y/6q48tFRgCfWVEQ
Da/fYIytUcIlppppUKfVUnI=
=C9dd
-----END PGP SIGNATURE-----