[Histonet] Best fixative for Mouse lung

[Elizabeth Chlipala]

Laurie

We would fix in either 10% NBF or paraformaldehyde.  We would inflate
the lungs with the fixative and then place the entire lungs in a
container with fixative.  You can do this with a blunt tipped syringe.
Carefully remove the sternum and dissect around the trachea and lungs,
you can leave the heart attached.  You do not want to knick the lungs or
you will not get good results.  For rats we would remove the lungs heart
and trachea in their entirety, you need to make sure you have a good
portion of the trachea present, we would then place the blunt end of the
needle into the trachea and tie the needle in place with suture, then we
would inflate the lungs with fixative and then remove the needle
carefully and tie off the trachea so the solution would remain in the
lungs and then place the lungs and heart in fixative.  For mice we would
do the same thing only that we inflated the lungs while they were in the
chest cavity.  Hope this helps.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)
Premier Histology Laboratory, LLC
P.O. Box 18592 
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
lizchlipala <@t> premierhistology.com
www.premierhistology.com
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Starkus,
Laurie
Sent: Thursday, February 19, 2004 10:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Best fixative for Mouse lung

For those of you doing rodent lung, what is your preferred fixative and
I
assume perfusion is your best method?

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