[Histonet] normal serum preparation

[Tony Henwood]

Nejat,

Collect the blood in a sterile 10ml plain centrifuge tube.
Allow to clot (5-10 minutes should do it)
Centrifuge 10min 1500rpm in a standard centrifuge
Collect supernatant (the serum)

Remember do not add anti-coagulant (eg EDTA, Citrate or Heparin). When you
centrifuge the blood, you will have plasma.

Ask for advice from your Haematology Department.

Regards

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: Nejat Yilmaz [mailto:nyilmaz <@t> mersin.edu.tr]
Sent: Tuesday, 17 February 2004 9:15 PM
To: histonet
Subject: [Histonet] normal serum preparation


Dear Histonetters...

Does anybody know how can I prepare the normal serum from fresh animal blood
for immunohistochemistry? 

Thanks in advance...
Dr. Necat Yılmaz
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
viruses.
**********************************************************************