[Histonet] Cutting 20-50 micron sections

[ricardo marcos]

I agree...I've worked with 30 micron paraffin liver sections...you
should try to cut with a very slow velocity, using a non disposable
microtome blade (I've used a plane wedge type) and warm the block, by
breathing on, as you cut every section.
Hope this helps.
Ricardo Marcos
Lab Histology and Embryology
ICBAS - Univ Porto, Portugal

-----Mensagem original-----
De: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Em nome de Tony
Henwood
Enviada: domingo, 15 de Fevereiro de 2004 22:14
Para: 'Featherstone, Annette'; Histonet (E-mail)
Assunto: RE: [Histonet] Cutting 20-50 micron sections

Annette,
Try warming the block up, in fact don't bother cooling the block at all.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html



-----Original Message-----
From: Featherstone, Annette [mailto:AFeatherstone <@t> KaleidaHealth.Org]
Sent: Friday, 13 February 2004 10:14 PM
To: Histonet (E-mail)
Subject: [Histonet] Cutting 20-50 micron sections


Any suggestions for cutting thick (20-50) micron paraffin sections. They
curl when they hit the blade and though I am perservering, I was hoping
for
a tip or two, or three or four or five .................
Annette FeatherstoneHT/MLT
Kaleida Health

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