[Histonet] mouse perfusion

[Charles Scouten]

You can get ALL the blood out, if sufficient pressure is used.  It is not about time or volume of fluid, but about pressure.  See the pictures at the following link.

http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=471001&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=Sacrifice+Equipment&idsubcategory=21

Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
www.myneurolab.com 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe
Sent: Friday, February 13, 2004 11:51 AM
To: Cooperman, Sharon (NIH/NICHD)
Cc: 'histonet <@t> lists.utsouthwestern.edu'
Subject: Re: [Histonet] mouse perfusion

Hi Sharon:

    You may be able to shorten the time of perfusion and avoid hand cramps by reducing the amount of washout solution. In my experience many people spend way too much time and solution washing blood out of the circulatory system. 10 ml of buffer or saline is plenty to wash the blood out of a mouse. After that it is on to fixative. You will never get all of the blood out of every vessel so why try.

Geoff

Cooperman, Sharon (NIH/NICHD) wrote:

>Dear Histoneteers,
>
>I am having trouble with mouse perfusion.  We do an intracardiac (left 
>ventricle perfusion).  I start out fine, but about halfway through the 
>perfusion I get a bad hand cramp (I have writer's cramp) and can't hold 
>the needle well.  I often wind up punching through the septum or the 
>anterior wall and the rest of the perfusion doesn't go well, so I get 
>good flushing of blood but poor fixation.  Does anyone know of a method 
>for holding the needle without hand fatigue?  There are people in my 
>lab who don't have writer's cramp who have much less trouble with 
>perfusions, but I can't change the writer's cramp stuff.
>
>Thanks,
>Sharon
>
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>  
>

--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************




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