Fwd: [Histonet] Critical Incident?

[Atoska S. Gentry]

Hello, I would suggest you rehydrate and process using appropriate 
solutions. The rehydration procedure that works well for me is from the 
Sheehan/Mosby 2nd or 3rd edition of Theory and Practice of Histotechnology. 
Please see as follows: place tissue in following solution overnight: 0.6g 
Sodium Carbonate, 42ml distilled H2O, 18m Absolute alcohol, and process the 
following day on a standard procedure, starting in 80% alcohol. Hope this 
helps. Atoska


>Date: Thu, 12 Feb 2004 14:25:54 -0500
>From: Derek and/or Lynda Leopold <leopold <@t> mnsi.net>
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>Hi Histonetters,
>     I am hoping to get some opinion off-list from supervisors (and anyone 
> else who may care to comment) on an event which happened recently in our 
> lab.  To be as short as possible, the person who was responsible for 
> changing the solutions on our Tissue Tek 5 biopsy processor somehow 
> (PUH-leese don't ask me how) made up the first alcohol to 70% (instead of 
> 95%), and the second alcohol to 88% (instead of 95%).  For context, we do 
> a short run, 20 minutes in each of formalin, 65% formal alcohol, then 2 
> changes of 95%, then 3 changes of 100% and on to xylene and 
> paraffin.  Now that you have the situation, I have two questions: 1) What 
> sorts of changes, if any,  would one expect in the biopsy tissues (endo, 
> cervix, etc) exposed to this miscalculated regimen ?  2) If, as some of 
> us surmise, this mistake in solution calculation could be linked to 
> biopsy tissues that were too dehydrated and brittle to be of any 
> diagnostic use, should that constitute a critical error/reportable mistake?
>    We have apparently never experienced such terrible tissues in recent 
> memory, and there is some debate as to whether the low-concentration 
> alcohols could have been the cause.
>I look forward to  your very expert opinions!
>Thanks
>Lynda Leopold
>Harrow, Ontario
>
>
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Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850