[Histonet] massons trichrome

[Rena Fail]

Incomplete washing after Weigert's will cause the purple staining. And
Make sure the Weigert's was correctly made. 
Rena Fail

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rita
Angel
Sent: Thursday, February 12, 2004 3:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] massons trichrome


hello all,

I was hoping someone could help us with a trichrome problem. We are 
suddenly having problems with the muscle in heart tissue staining purple

instead of red. Our skin control is staining the same way. There are 2 
techs in the lab, and we're both having problems. The problems are with 
complete batches, not slides within the batch. We each stained 2 batches
in 
a row, one batch stained with the purple muscle, and the 2nd batch with
red 
muscle. We're leaving the slides in the solutions the same amount of
time 
and rinsing the same amount of times, etc. We're using the same
solutions 
from the same bottles, etc. (we make all of our own solutions). We are 
unable to think of what could be causing the problem. Also, our slides
are 
still really black when they are finished rinsing after the wiegert's
step. 
Could this be causing it? I appreciate any advice.

Thanks,

Rita Angel, HT
University of Cincinnati


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