[Histonet] massons trichrome
Rena Fail
RFail <@t> Charleston.net
Thu Feb 12 16:54:52 CST 2004
Incomplete washing after Weigert's will cause the purple staining. And
Make sure the Weigert's was correctly made.
Rena Fail
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rita
Angel
Sent: Thursday, February 12, 2004 3:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] massons trichrome
hello all,
I was hoping someone could help us with a trichrome problem. We are
suddenly having problems with the muscle in heart tissue staining purple
instead of red. Our skin control is staining the same way. There are 2
techs in the lab, and we're both having problems. The problems are with
complete batches, not slides within the batch. We each stained 2 batches
in
a row, one batch stained with the purple muscle, and the 2nd batch with
red
muscle. We're leaving the slides in the solutions the same amount of
time
and rinsing the same amount of times, etc. We're using the same
solutions
from the same bottles, etc. (we make all of our own solutions). We are
unable to think of what could be causing the problem. Also, our slides
are
still really black when they are finished rinsing after the wiegert's
step.
Could this be causing it? I appreciate any advice.
Thanks,
Rita Angel, HT
University of Cincinnati
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
More information about the Histonet
mailing list