[Histonet] IHC background stain

Thu Feb 12 13:11:04 CST 2004

Hello all,  
I do all the  IHC for our diagnostic veterinary laboratory.  Latley i have been getting a lot of background stain from certain protocols.  I also over heard one of the pathologists state the sections are getting thicker.  Could thick section cause background stain on IHC?  I checked my histologist microtome and it is set at 5um. Could her microtome need calibrated?

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808