[Histonet] IHC on H&E section

[GUTIERREZ, JUAN]

A variation on this theme is using a cigarrette lighter to "melt" the mountant and then pop-off the coverslip with a razor blade.  It takes about a minute per slide.  Good luck,
Juan
 
p.s. You can usually see an air bubble form under the coverslip when is ready to be popped-off.
 

	-----Original Message----- 
	From: Mary North [mailto:northma <@t> ohsu.edu] 
	Sent: Wed 2/11/2004 11:54 AM 
	To: pruegg <@t> colobio.com; histonet <@t> pathology.swmed.edu 
	Cc: ihcrg <@t> yahoogroups.com 
	Subject: Re: [Histonet] IHC on H&E section
	
	

	When working with a large tumor registry, I was asked to do IHC on many
	archived H & E slides.  To remove the coverslip, I placed the slide on a
	hot plate until the adhesive softened and then popped off the coverslip
	by slipping a razor blade under one corner.  No tissue was ever lost.
	While the slide was still slightly warm, I put it into xylene to remove
	the remaining adhesive. Then I continued down a typical decerating line
	to 70% ethanol which removed most of the eosin.  To remove the
	hematoxylin, I used periodic acid solution(I don't remember the
	concentration or time but I think it was similar to the PAS treatment).
	The pathologist was found that the IHC's on the archived slides where
	much more brilliant than on freshly cut sections.
	Mary North, HT(ASCP), HTL
	Oregon Health & Science University
	Portland, OR
	
	>>> "Patsy Ruegg" <pruegg <@t> colobio.com> 02/11/04 07:36AM >>>
	I know this has come up before but don't remember the details.  I would
	like
	to do IHC for AE1/AE3 on a slide from a study set that has been H&E
	stained
	and coverslipped for some time now.  Please advise.  Is there a
	preferred
	method for removing the coverslip and maintaining the section integrety?
	Destaining the H&E?  Doing the IHC stain on this section?
	Patsy
	
	
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