[Histonet] IHC on H&E section

[Gary Gill]

Unless the oven's explosion-proof, heating xylene is a potential
flammability/explosion hazard -- notwithstanding that the Coplin jar is
covered.

Gary Gill

-----Original Message-----
From: DiCarlo, Margaret [mailto:MDiCarlo <@t> KaleidaHealth.Org] 
Sent: Wednesday, February 11, 2004 11:29 AM
To: 'Patsy Ruegg'; Histonet <@t> Pathology. Swmed. Edu
Cc: Ihcrg <@t> Yahoogroups. Com
Subject: RE: [Histonet] IHC on H&E section


Patsy,

To make the coverslip come off faster, fill a coplin jar with xylene and put
the slides in, cover with a top and then put the coplin jar into a 60 degree
C. oven and agitate the xylene occaisionally and the coverslip will come off
in much less time than soaking at room temperature. It's a little trick I've
learned.

Peggy DiCarlo HT(ASCP)

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> colobio.com]
Sent: Wednesday, February 11, 2004 10:36
To: Histonet <@t> Pathology. Swmed. Edu
Cc: Ihcrg <@t> Yahoogroups. Com
Subject: [Histonet] IHC on H&E section


I know this has come up before but don't remember the details.  I would like
to do IHC for AE1/AE3 on a slide from a study set that has been H&E stained
and coverslipped for some time now.  Please advise.  Is there a preferred
method for removing the coverslip and maintaining the section integrety?
Destaining the H&E?  Doing the IHC stain on this section? Patsy


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

CONFIDENTIALITY NOTICE: 
This email transmission and any documents, files, 
or previous e-mail messages attached to it are 
confidential and intended solely for the use of the 
individual or entity to whom they are addressed. 
If you are not the intended recipient, or a person 
responsible for delivering it to the intended recipient, 
you are hereby notified that any further review, 
disclosure, copying, dissemination, distribution, or 
use of any of the information contained in or attached 
to this e-mail transmission is strictly prohibited. 
If you have received this message in error, please 
notify the sender immediately by e-mail, discard 
any paper copies, and delete all electronic files 
of the message. If you are unable to contact the 
sender or you are not sure as to whether you 
are the intended recipient, please e-mail 
ISTSEC <@t> KaleidaHealth.org or call (716) 859-7777. 



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet