[Histonet] IHC on H&E section

[Stapf, Ross]

In my experience destaining is not necessary if you are doing a heat
epitope retrieval.  The retrieval will remove most if not all of the H/E
stain.  You may have a slight blush of eosin when finished, but it will
be easy to distinguish from DAB staining in my experience.  I have
recently heard that there are some antibodies that may not be preserved
after H/E staining, but I have not experienced this personally.  AE1/AE3
should be fine.

Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465
214-820-4110 fax
RossS <@t> baylorhealth.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne
Mauger
Sent: Wednesday, February 11, 2004 10:00 AM
To: pruegg <@t> colobio.com; histonet <@t> pathology.swmed.edu
Cc: ihcrg <@t> yahoogroups.com
Subject: Re: [Histonet] IHC on H&E section


Patsy,
>From my experience it is possible. I would soak the slide in fresh
>xylene,changing often, until it will lift off without lifting the
tissue.
The H&E stain can be somewhat lightened with acid alcohol, and 95% alc.
The destaining is not really necessary. Depending on the
antigen,hopefully it is preserved, the IP staining should work as
usual,with a little dirty H&E background. Good luck. Sometimes it takes
days for coverslips to come off. Jo

>>> "Patsy Ruegg" <pruegg <@t> colobio.com> 02/11/04 10:36AM >>>
I know this has come up before but don't remember the details.  I would
like to do IHC for AE1/AE3 on a slide from a study set that has been H&E
stained and coverslipped for some time now.  Please advise.  Is there a
preferred method for removing the coverslip and maintaining the section
integrety? Destaining the H&E?  Doing the IHC stain on this section?
Patsy


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