[Histonet] over fixing problem

Geoff McAuliffe mcauliff <@t> umdnj.edu
Wed Feb 11 13:01:49 CST 2004

Hi Loralee:

    Exactly what is the problem with your overfixed tissues? You said 
that they cut well so ....... Glut fixed tissue does react "abnormally" 
with some stains, when compared to formalin fixed material. Is that the 
problem? There are ways to block the aldehyde groups that remain in glut 
fixed tissue, treatment with sodium borohydride in phos. buffer comes to 
mind. I can dig up a recipe if you need one.
    By the way, I have heard the term "overfixed" for many years. I am 
not really sure that, other than making tissue harder to section,  if 
there really is such a thing for routine light microscopy.



> I have some samples that were fixed in a rather strong concentration 
> of gluteraldehyde.  I know that they are extrememly overfixed.  I have 
> cut them with no problem.  I am wondering if there is something that I 
> can do to the sections to help with the overfixation.    Any advice 
> would be appreciated.
> Loralee Gehan
> Orthopaedics Research Lab
> University of Rochester
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu

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