[Histonet] RE: [IHCRG] apoptosis vs necrosis

[Luis Chiriboga]

check out the following article "comparison of IHC for Activated caspase3
and cleaved cytokeratin 18 with the tunel method for quantification of
apoptosis in histological sections of PC-3 Subcutaneous xenografts" J Path
199:221-228 2003

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> colobio.com]
Sent: Thursday, February 05, 2004 3:47 PM
To: Bruijntjes, J.P.; histonet <@t> lists.utsouthwestern.edu
Cc: Ihcrg <@t> Yahoogroups. Com
Subject: [IHCRG] apoptosis vs necrosis


I am charged with trying to label apoptotic cells in mouse tissue that is
necrotic due to the bacterial infection it has been exposed to.  I have
previously worked extensively with the tunnel assay for apoptosis and could
not ever tell the difference between apoptotic cells and necrotic cells.  Is
there something new that would make this distinctsion?  Please advise

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Bruijntjes, J.P.
Sent: Thursday, February 05, 2004 1:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)


Hi all

Is anyone of you familiar with a marker for apoptosis called "Cytodeath"
(M30) from Roche on rat tissues?

Joost Bruijntjes
TNO Nutrition and Food Research
PO Box 360
3700 AJ
Zeist
The Netherlands


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