[Histonet] immunofluorescent antibody tissue controls

[Garrison, Becky]

Linda, 
Since we on an immunofluorescent thread...would any one
be willing to share their protocol for IF on kidney biopsies
(IgG, IgA, IgM, C3) including source/dilutions of antibodies?
Thanks.

Becky Garrison
Pathology
Shands Jacksonville
904-244-6237

-----Original Message-----
From: LINDA MARGRAF [mailto:LINDA.MARGRAF <@t> childrens.com]
Sent: Thursday, February 05, 2004 1:27 PM
To: Histonet <@t> lists.utsouthwestern.edu
Cc: MICHELLE MCNEESE
Subject: [Histonet] immunofluorescent antibody tissue controls


Dear Histonetters
We are continually running low on control tissue for our
immunofluorescent (IF) stains for IgG, IgA, IgM, and C3 that we run on
kidney biopsies.  Our pediatric population rarely has a kidney removed
for diseases with antibody deposits and none of our neighboring
hospitals has any control tissue to spare. DOes anyone know of a good
commercial source for positive IF control tissue for Igs and C3?
Thanks
Linda M
Histonet administrator
Director of Anatomic Pathology
Children's Medical Center of Dallas


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