[Histonet] marking ink

[Favara, Cynthia (NIH/NIAID)]

Sharon,

I have tried numerous inking dyes for mouse brain and spinal cord and have
found that they obscure the stain we use here to a significant extent. I
have found that the best thing to do it to get a very good pair of
magnifying glasses and familiarize yourself with the anatomy. I also place
the spinal cord on tissue/lens paper with a specified side down. Hope this
helps.
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: Cooperman, Sharon (NIH/NICHD) 
Sent: Tuesday, February 03, 2004 2:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] marking ink


Dear Histonetters,

I'm also looking for marking ink, something I can use to mark the 
orientation of a mouse spinal cord (dissected out of the spine) to 
tell which side is dorsal or ventral to orient myself for embedding. 
Does anyone know of something that would work for this?

Thanks,
Sharon
-- 
Sharon Cooperman        	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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