[Histonet] Improper tissue processing

[Marion Hiles]

Thats very odd. How do you know the last 100% alcohol was actually 87%? We
have a VIP E300.How often do you replace the reagents and do you bump up the
reagents, so that the last of, say, the 3 100% alcohols is the freshest?

Bob Quilty
Neuropath
Frenchay Hospital
Bristol UK

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Kari
Breal
Sent: 28 December 2004 19:27
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Improper tissue processing


I have had another tissue malfunction.  The last time was July 4th weekend
and it happened again last weekend- Christmas weekend.  The tissue came off
of the processor mushy- I suspect with water still in them.  The processor
did not malfunction or error according to the blank error log- we have a VIP
E300.  When I checked the reagents in the processor- the reagents up to the
last 100% were correct.  The last 100% alcohol was at 87%.  Which to me is
what caused the raw tissue but what I can not figure out is how it got that
way.  This happened on Friday with minimal staff.  No one changed any
reagents on Friday and the tissue from Friday morning was normal.  The
processor was due to be rotated on Monday the 27th. 

My doc's are looking for reasons and answers and I am drawing a blank.  I
was hoping another histologist outside of this chaos might be able to think
of questions or reasons that I am not currently able to.

I also would like to know how everyone is treating their under processed
tissue.  Reprocessing by hand? Reprocessing on the VIP? We actually
reprocessed using a microwave processor.  The tissue turned from mush to
extremely hard tissues.  Unfortunately 45 of the 50 blocks were prostate
bx's and there was very little tissue to begin with.

Thank you for your assistance!

Kari Breal
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