[Histonet] RE: Histonet Digest, Vol 13, Issue 33

Tue Dec 28 13:19:45 CST 2004

Ole,
The procedure for the Sevier-Munger can be found on page 257 of Theory and
Practice of Histotechnology by Sheehan, Hrapchak, 1980.  If you don't have
this book, I'd be happy to fax the procedure to you or send it email.

Julie

Julie Sanders, BA, HT(ASCP)
Supervisor, Anatomic Pathology
VAMC, Cincinnati, Ohio 
------------------------------

Message: 11
Date: Tue, 28 Dec 2004 16:29:34 +0100
From: "Ole" <osteffe <@t> online.no>
Subject: [Histonet] Sevier Munger Silver stain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000e01c4ecf2$0a2c9070$0100000a <@t> brukere>
Content-Type: text/plain;	charset="iso-8859-1"

Hi

I need a protocol/receipe of the Sevier Munger silver stain. I have not
found it in any of my histotech books nor had any luck searching the web. I
work with PCR, immunohistochemistry and in situs and dont know much about
special stains, or where to find them, if their not in our "special stain"
books.
It is to be used on Formaldehyd fixed paraffin embedded tissue.

Best regards
Ole

------------------------------

Message: 12
Date: Tue, 28 Dec 2004 07:36:46 -0800 (PST)
From: Gareth Davis <mrsgbd2001 <@t> yahoo.com>
Subject: Re: [Histonet] please advise: bor practical
To: lpwenk <@t> sbcglobal.net, Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20041228153646.66909.qmail <@t> web52705.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

thanks for information on the HT Practical.  I did realize that there were
other criteria involved in the grading, but most of my comments were on
staining.  Anyway, I thought about taking the HTL, but it seems most people
I know only have an HT and are doing almost as well in the field.
Thanks again,
Gareth Davis

lpwenk <@t> sbcglobal.net wrote:
Just wondering - Did you take the HTL exam and the rest of the people take
the HT exam? Since you have a BS?

The HTL exam IS harder than the HT exam - larger tissues sections, trickier
stains, often needing to cut thinner tissues, tissues that are more
difficult to obtain, etc.

I train both HT and HTL students, and can attest that the HTL exam is harder
to do than the HT exam.

Peggy A. Wenk, HTL(ASCP)SLS
Program Director, Schools of Histotechnology
William Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
From: "Gareth Davis" 
To: ; "Histonet" 
Sent: Monday, December 27, 2004 5:48 PM
Subject: Re: [Histonet] please advise: bor practical

> Hey Joan,
> I'm in the exact same boat as you. Our lab does all
> work manual except the H&E stain. There were seven of
> us, from my lab, taking the test this fall. Out of the
> seven I was the only one to fail. (I am also sending
> my slides in for review.) I don't see how I could
> have failed - based on the staining of three of my H&E
> slides - when I used the same H&E machine my
> co-workers used. Not that a degree gives a person
> more "talent", but I am the only one of the seven with
> a B.S., and I wonder if that they scored me on a
> higher scale - which would not seem fair. I was told
> that the evaluation process was anonymous, by a member
> of NSH.
> I would also be interested in what happens with the
> reevaluation process.
>
> Good luck to you.
> Gareth
>
> --- Joanholtz <@t> aol.com wrote:
>
> > The hospital in which I work uses manual processes
> > for all of our cutting and
> > special staining needs. The only automation we use
> > is the H&E. I would
> > think this is the preferred way to learn hands on
> > and was quite proud to learn
> > this way. I was assured that after passing the
> > written portion of the exam,
> > (which I did on my first attempt) that the practical
> > should be no problem at all.
> > I have submitted my practical twice; this time I am
> > eligible to submit my
> > slides for a reevaluation along with more money.
> > Before each submission my
> > slides were reviewed by at least two of our
> > pathologists. If 800 is the optimal
> > score I do not think it is reasonable to assume that
> > my hospitals lab puts out
> > or accepts anything below a 300. I would truly
> > appreciate any and all help or
> > insights as I try and appeal my scores by Jan. 10th
> > 2005.
> >
> > Am I at a disadvantage without automation? How
> > should I select slides to be
> > reevaluated? Is it possible to get a worse score?
> > Am I wasting my time? Is
> > the evaluation process anonymous, do they know your
> > background?
> >
> > This is truly a very frustrating situation with a BS
> > in Biology and three
> > years histology experience. I have invested a lot of
> > time, energy and money into
> > getting where I am now and this arbitrary process is
> > preventing me from going
> > any further.
> >
> > Thank you for any and all help,
> > Joan
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> >
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
>
>
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------------------------------

Message: 13
Date: Tue, 28 Dec 2004 07:41:30 -0800
From: "Robyn Vazquez" <vazquezr <@t> ohsu.edu>
Subject: Re: [Histonet] Sevier Munger Silver stain
To: histonet <@t> lists.utsouthwestern.edu,	osteffe <@t> online.no
Message-ID: <s1d10e36.005 <@t> gwsmtp.ohsu.edu>
Content-Type: text/plain; charset=us-ascii

Ole,
Do you have fax no.?  I have recipe for Sevier-Munger Method for neural
tissues

Robyn
Ohsu

>>> "Ole" <osteffe <@t> online.no> 12/28/2004 7:29:34 AM >>>

Hi

I need a protocol/receipe of the Sevier Munger silver stain. I have not
found it in any of my histotech books nor had any luck searching the
web. I work with PCR, immunohistochemistry and in situs and dont know
much about special stains, or where to find them, if their not in our
"special stain" books.
It is to be used on Formaldehyd fixed paraffin embedded tissue.

Best regards
Ole
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 14
Date: Mon, 27 Dec 2004 10:44:38 -0800
From: "FIGUEIREDO,MARXA L        " <mlfiguei <@t> ucla.edu>
Subject: [Histonet] help
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1104173078.41d05816c1428 <@t> mail.ucla.edu>
Content-Type: text/plain; charset=ISO-8859-1

Hi HIstonetters,

Has anyone done imunohistochemisty with anti-GFP in paraffin tissues
recently? I have the anti-GFP monoclonal from Clontech (JL-8) and was
wondering if anyone has tried that one in FFPE tissues. If so, what was
the dilution and antigen retrieval method?
THanks

Marxa Figueiredo
UCLA Dentistry

------------------------------

Message: 15
Date: Tue, 28 Dec 2004 09:54:54 -0600
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
Subject: RE: [Histonet] Sevier Munger Silver stain
To: "Ole" <osteffe <@t> online.no>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<A61F23E937E4DA488E0C0F60093843D901E49802 <@t> ccetxm030.echristus.net>
Content-Type: text/plain;	charset="iso-8859-1"

It's in the AFIP manual.  The reference is; Sevier, A. C., and Munger, B.
L.: J. Neuropath. Exp. Neurol. 24:130-135, 1965.
Sorry but I don't have time to type the whole thing.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long live
free speech!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ole
Sent: Tuesday, December 28, 2004 9:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sevier Munger Silver stain

Hi

I need a protocol/receipe of the Sevier Munger silver stain. I have not
found it in any of my histotech books nor had any luck searching the web. I
work with PCR, immunohistochemistry and in situs and dont know much about
special stains, or where to find them, if their not in our "special stain"
books.
It is to be used on Formaldehyd fixed paraffin embedded tissue.

Best regards
Ole
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 16
Date: Tue, 28 Dec 2004 11:44:28 -0500
From: MTitford <@t> aol.com
Subject: [Histonet] On passing BOR exam
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <3F49D0EC.73796666.00762DB1 <@t> aol.com>
Content-Type: text/plain; charset=iso-8859-1

Joan Holtz asks about passing the BOR exam.

I was shocked a few years ago when I read the ASCP newsletter that gave
statistics for the registry exams. Many histotechs failed the HT and HTL
practicals.
Now, not talking about Joans laboratory or anyone else's in particular, I
suspect many applicants bang out their exam slides like they do their daily
work slides and send them off without critically examining them first.
In our laboratory we receive slides for consultation which are sub-standard.
Obviously those laboratories think they are acceptable.
With HT and HTL practical exam slides you should produce the best d***
slides you can! You have plenty of time. Scrounge for the best fixed
tissues. Submit tissues of right size allowing for shrinkage. Process
correctly. Cut correctly. Produce best stained slides you can. In histology,
it is easy to produce adequate slides, but hard to produce perfect
slides.The slides should be of right thickness, no scores, folds,
precipitates, background staining etc. The staining should be perfect!
Remember, if you submit a poorly stained slide, you are telling the examiner
thats you best work.
In my laboratory when preparing for practical exams, I expect histotechs to
make multiple blocks, and multiple stained slides from those blocks. We sit
down together several times and look closely at those slides. There is much
going back and re-submitting tissue. We have only had one histotech fail the
practical in 25 years (And that tech did not want me to see those slides!)

Mike Titford
USA Pathology
Mobile AL USA

------------------------------

Message: 17
Date: Tue, 28 Dec 2004 11:59:02 -0500
From: MTitford <@t> aol.com
Subject: [Histonet] Argyrophil stains.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49AF28FD.5B6B2BA3.00762DB1 <@t> aol.com>
Content-Type: text/plain; charset=iso-8859-1

Ole, somewhere overseas I suspect, asks about the Sevier Munger stain.
The reference is: Sevier, A.C., Munger, B.L., A silver method for paraffin
sections of neural tissue. J. Neuropath Exp Neurol. 24: 130 - 135 1965
However, in the USA (and is it not interesting how labs use procedures
written by their own countrymen?)many labs use the Churukian method which
demonstrates a wider variety of argyrophil tumors. Churukian, J.,Schenk,
E.A. A modification of Pascual's argyrophil method. J. Histotechnol 3: 102 -
102 1979.
I read somewhere, long ago, that there is no one method which will
demonstrate ALL argyophil tumors, but the Chrukian method demonstrates the
widest variety.

Mike Titford
USA Pathology
Mobile AL USA

------------------------------

Message: 18
Date: Tue, 28 Dec 2004 12:32:48 -0500
From: "Favara, Cynthia (NIH/NIAID)" <cfavara <@t> niaid.nih.gov>
Subject: RE: [Histonet] help
To: "'FIGUEIREDO,MARXA L        '" <mlfiguei <@t> ucla.edu>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<F76C9B2DA2FC4C4CA0A18E288BBCBCF70A8DB103 <@t> nihexchange24.nih.gov>
Content-Type: text/plain

I have been doing anti GFP in mouse FFPE tissue. I am presently using
Molecular Probes Rabbit polyclonal 2mg/ml at a dilution of 1:500 using a
biotinylated secondary from Vector @1:250 dil on the Ventana Nexus. I use
Citrate pH 6.0 in a pressure cooker temp 120 for 30 seconds. I will be
evaluating monoclonal from Zymed and BD Biosciences  when I get the time.
Sorry I have no experience with Clontech.

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: FIGUEIREDO,MARXA L [mailto:mlfiguei <@t> ucla.edu] 
Sent: Monday, December 27, 2004 11:45 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] help

Hi HIstonetters,

Has anyone done imunohistochemisty with anti-GFP in paraffin tissues
recently? I have the anti-GFP monoclonal from Clontech (JL-8) and was
wondering if anyone has tried that one in FFPE tissues. If so, what was the
dilution and antigen retrieval method?
THanks

Marxa Figueiredo
UCLA Dentistry

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 19
Date: Tue, 28 Dec 2004 11:49:32 -0600
From: "Charles.Embrey" <Charles.Embrey <@t> carle.com>
Subject: RE: [Histonet] On passing BOR exam
To: <MTitford <@t> aol.com>,	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<C320919A1C432845938BCD8CC3294A30205D95 <@t> EXCHANGEBE1.carle.com>
Content-Type: text/plain;	charset="us-ascii"

Thank-you! Thank-you! Thank-you!  I am shocked by the laid-back attitude
that many people today take in approaching the practical exam.  They
don't seem to even read the grading criteria required to produce the
slides capable of passing.  Each slide sent in to be graded should first
be graded by the technician against these grading guidelines.  There
should really be no surprises.  If you don't know enough about histology
to grade your own slides you really shouldn't be certified as a
histotech.  As far as automation goes I think all practical exam slides
should be produced WITHOUT automation.  I don't ever want to hire a
histotech that can't do a GMS by hand. Sorry if this seems harsh but as
a lab manager I want to see the BOR stamp of approval mean something.
Quit wringing your hands; take a look at the list of errors provided by
the grader and produce slides that avoid those mistakes.
Charles Embrey, Pathologists' Assistant, HT(ASCP)
Histology Manager
Carle Clinic, Urbana Illinois  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
MTitford <@t> aol.com
Sent: Tuesday, December 28, 2004 10:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] On passing BOR exam

Joan Holtz asks about passing the BOR exam.

I was shocked a few years ago when I read the ASCP newsletter that gave
statistics for the registry exams. Many histotechs failed the HT and HTL
practicals.
Now, not talking about Joans laboratory or anyone else's in particular,
I suspect many applicants bang out their exam slides like they do their
daily work slides and send them off without critically examining them
first.
In our laboratory we receive slides for consultation which are
sub-standard. Obviously those laboratories think they are acceptable.
With HT and HTL practical exam slides you should produce the best d***
slides you can! You have plenty of time. Scrounge for the best fixed
tissues. Submit tissues of right size allowing for shrinkage. Process
correctly. Cut correctly. Produce best stained slides you can. In
histology, it is easy to produce adequate slides, but hard to produce
perfect slides.The slides should be of right thickness, no scores,
folds, precipitates, background staining etc. The staining should be
perfect! Remember, if you submit a poorly stained slide, you are telling
the examiner thats you best work.
In my laboratory when preparing for practical exams, I expect histotechs
to make multiple blocks, and multiple stained slides from those blocks.
We sit down together several times and look closely at those slides.
There is much going back and re-submitting tissue. We have only had one
histotech fail the practical in 25 years (And that tech did not want me
to see those slides!)

Mike Titford
USA Pathology
Mobile AL USA

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

_______________________________________________
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End of Histonet Digest, Vol 13, Issue 33
****************************************