[Histonet] help

[Favara, Cynthia (NIH/NIAID)]

I have been doing anti GFP in mouse FFPE tissue. I am presently using
Molecular Probes Rabbit polyclonal 2mg/ml at a dilution of 1:500 using a
biotinylated secondary from Vector @1:250 dil on the Ventana Nexus. I use
Citrate pH 6.0 in a pressure cooker temp 120 for 30 seconds. I will be
evaluating monoclonal from Zymed and BD Biosciences  when I get the time.
Sorry I have no experience with Clontech.

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: FIGUEIREDO,MARXA L [mailto:mlfiguei <@t> ucla.edu] 
Sent: Monday, December 27, 2004 11:45 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] help

Hi HIstonetters,

Has anyone done imunohistochemisty with anti-GFP in paraffin tissues
recently? I have the anti-GFP monoclonal from Clontech (JL-8) and was
wondering if anyone has tried that one in FFPE tissues. If so, what was the
dilution and antigen retrieval method?
THanks

Marxa Figueiredo
UCLA Dentistry

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