[Histonet] Re: Histonet Digest, Vol 13, Issue 30

[Amos Brooks]

Hi,
         I have found that potassium permanganate usually at least weakens 
a signal, so if you already have a weak signal you may have difficulty with 
it. The bigger problem is that sections love to fall off or get torn up 
when this is used. Even if you use silanized slides you are bound to 
experience some section loss. I wish there were a better way to remove 
melanin but I've not seen any that give any better results without the same 
problems. My advice: use low concentrations for a longer time and proceed 
with caution.
Best of Luck,
Amos Brooks


At 01:01 PM 12/23/2004, you wrote:
>Message: 2
>Date: Wed, 22 Dec 2004 13:15:06 -0500
>From: "Kristopher Kalleberg" <Kristopher.Kalleberg <@t> unilever.com>
>Subject: [Histonet] melanin bleaching
>To: "histonet <@t> lists.utsouthwestern.edu"
>         <histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <ISSMTP.2000_38_.20041222131506.224F <@t> unilever.com>
>Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
>
>Can someone tell me what the best way to go about bleaching melanin would 
>be if
>I was trying to find label for catalase?  Will the potassium permanganate
>destroy the antigenicity.