[Histonet] rabbit bone specimens[Scanned]

[Kemlo Rogerson]

I would have used celloidin and low viscosity nitrocellulose embedding for
such things as eyes and bones. But I'm sure this 'old' technique has been
superseded by a posher new technic.

If not, Lillie has a good section on it on page 92 "Histopathologic Technic
and Practical Histochemistery". If you can't find it or are unable to find
anything better then send me an e-mail. 

Kemlo Rogerson
Cellular Pathology Manager
East Lancashire Hospitals NHS Trust
DD. 01254-294162
Mobile 0774-9754194
 

-----Original Message-----
From: Mary Lloyd [mailto:lloyd.3 <@t> osu.edu] 
Sent: 16 December 2004 16:25
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] rabbit bone specimens[Scanned]

I am working on a research projects with rabbit femurs and tibias.  They 
are decalcified in cal-ex from fisher for 2 weeks and then processed for 
1.5 hours in each solution on the processor.  2 changes of 70, 95, 100%, 
xylene and then paraffin.  I am really having problems with the cartilage 
folding right at the curve of the specimen.  It seems as though the bone 
just below the cartilage is not thoroughly processed or  hard. When I do 
feel I get a decent section it seems to fold when I stain.  I am using plus 
slides. I don't normally do a lot of research so if anyone can help me with 
this problem either with cutting or processing solutions I would really 
appreciate the help.


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