[Histonet] CD34 and CD31 in paraffin mouse tissue

[Elizabeth Chlipala]

Michelle

It also works on rat.  I have tried it on formalin perfused rat brains
and it works.  I use steam retrieval for 20 minutes, antibody at 1:500,
30 minutes RT, a rabbit anti-goat from vector at 1:400 and then rabbit
envision from Dako to amplify.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: Michelle McDonald [mailto:MichelM9 <@t> chw.edu.au] 
Sent: Wednesday, December 15, 2004 2:06 PM
To: 'Elizabeth Chlipala'; wasielewski.reinhard.von <@t> mh-hannover.de;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD34 and CD31 in paraffin mouse tissue

Dear Liz,

Would you know whether this antibody from Santa Cruz would work on rat
tissue. I am currently trying to get a CD31 antibody from Calbiochem to
work
on FFPE rat bone sections (decalcified) and I am having no luck. Can you
suggest an antibody I could try or recommend an antigen retrieval
technique.
I have tried Proteinase K and Pepsin.

Thank you in advance for your advice

Michelle 

Michelle McDonald B.MedSci
 
PhD Student
Research Assistant
Orthopaedic Research Dept.
The Children's Hospital Westmead.
Westmead NSW 2145
Australia
 
Ph. +612 9845 3087
Fax. +612 9845 3078
 


-----Original Message-----
From: Elizabeth Chlipala [mailto:liz <@t> premierlab.com] 
Sent: Thursday, 16 December 2004 2:52 AM
To: wasielewski.reinhard.von <@t> mh-hannover.de;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD34 and CD31 in paraffin mouse tissue


Reinhard

Santa Cruz has a goat polyclonal for CD-31 (PECAM-1) that will work in
FFPE
tissue with antigen retrieval. Cat SC-1056.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
wasielewski.reinhard.von <@t> mh-hannover.de
Sent: Wednesday, December 15, 2004 7:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD34 and CD31 in paraffin mouse tissue

Hello Histonetters,
we are looking to immunostain endothelial cells (vessels) in FFPE mouse
tissue. 
There is no cross-reactivity to the human epitopes, therefore the
antibodies
for 
human tissue don't work (we have tried it). Is there anyone out there
who
can help us 
? (Clones, suppliers, ...)
Answers wanted :-)

Best wishes for the rest of this year

Reinhard von Wasielewski


PD Dr. med. Reinhard von Wasielewski


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