[Histonet] CD 68 on murine brain

[Elizabeth Chlipala]

Cynthia

I use serotec's antibody F4/80 for macrophages in mouse tissues. I have
not tried it on brain, but I even have done it on formalin fixed, formic
acid decalcifed mouse joints. This antibody will work on FFPE or
paraformaldehye fixed tissue with proteinase K digestion.  Here is the
basic protocol.

1:1000 primary 30 minutes room temp
Proteinase K  pretreatment - 5 minutes
Rabbit anti-rat 1:400 30 minutes
Envision Rabbit - 30 minutes

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Favara,
Cynthia (NIH/NIAID)
Sent: Monday, December 13, 2004 12:54 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD 68 on murine brain

Has anyone done any CD68 oh FFPE mouse brain?

I will search the archives and do a lit search but have not as of this
time,
Thanks,
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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