[Histonet] H and E vs. Masson Trichrome in cardiac injury

[Molinari, Betsy]

Hi Natalie,
We use Massons Trichrome for all our cardiac fibrosis studiea, paraffin
and frozen. I a 0.1% light green counterstain. I am not involved in the
evaluation end of the studies but will try and get that information and
let you know.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
Houston,TX 77030
832-355-6524

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Lapointe, Nathalie,Ph.D.
Sent: Wednesday, December 08, 2004 12:24 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] H and E vs. Masson Trichrome in cardiac injury


> Hi,
> 
> I found that website by hazard and I'm very impress!
> 
> I have another questions for you:
> 
> I'm currently exploring some hormones that may cause myocardial injury
in
> adrenalectomized rats. 
> The heart were stained with hematoxylin and eosin for light
microscopic
> analysis (x40). A scale from 0-4 was used to score the level of
myocardial
> injury. A score of 0 represented no damage. A score of 1 represented
the
> presence of myocytes demonstrating early necrotic changes such as
nuclear
> pyknosis or karylysis, and oesinophil staining of the cytoplasm
associated
> with the presence of scattered neutrophilic infiltrates.  A score of 2
was
> given when one clear area of necrosis (loss of myocardial cells with
heavy
> neutrophilic infiltrates) was observed.  When two or more separate
areas of
> necrosis were found (implicating the presence of two different
myocardial
> infarctions in the same heart), but the areas localized and
compromised less
> then 50% of the ventricular wall, the hearts received a score of 3. A
score of
> 4 was assigned to hearts that demonstrated extensive areas of necrosis
> compromising more than 50% of wither the left or the right ventricle. 
> 
> I'm looking for another way then the above score to measure cardiac
injury.
> Based to the fact that Hematoxylin is a dark purplish dye that will
stain the
> chromatin within the nucleus (therefore also monocyte infiltration),
and that
> Eosin is an orangish pink to red dye that stains the cytoplasmic
material
> including connective tissue and collagen (therefore doesn't stain
necrotic
> tissues), I was wondering if a software in which I could measure the
contrast
> of color related to injury vs. intact tissue exist? Does this same
software
> (if available) can also calculated the number of dark purplish dye
(therefore
> level of inflammation)?
> 
> We also stained the same heart with Masson Trichrome. Do you think
that
> measuring the % of blue staining (collagen) in the whole heart at a
> magnification of X4 is equivalent to H and E score described above?
How can I
> know if the blue staining with Masson Trichrome is fibrosis rather
then
> collagen or only red Sirus staining can measure fibrosis? 
> 
Thank you very much for all your help.

Best,

> Nathalie Lapointe
> Brigham and Women's Hospital
> LMRC #214
> 221 Longwood Ave
> Boston, MA, USA
> 02115
> (617) 515-5936
> 
> 

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