[Histonet] RE: static electricity/humidity problems
Philip Oshel
peoshel <@t> wisc.edu
Fri Dec 3 08:28:22 CST 2004
"Cardinal"? Do you have a website for this company? I've been looking
for a supplier of molecular sieve that doesn't charge outrageous
prices.
I assume the numbers are the catalog number for the sieve -- is it 3
- 4 Angstrom? The sieve pores must be that size to trap water
molecules.
Thanks.
Phil
>And Cardinal has a product called Molecular Sieve - 4494-500ny that
>works the same way without the carcinogen factor. (At least as far
>as I know!)
>Joyce
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
>bettina.hutz <@t> orionpharma.com
>Sent: Friday, December 03, 2004 2:52 AM
>To: rgrow <@t> bmnet.com; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] RE: static electricity/humidity problems
>
>
>Hello Renee,
>
>this sounds good, I WILL try it:)
>Thanks a lot, "kiitos" as we say in finnish:)
>Tina
>
>-----Original Message-----
>From: rgrow <@t> bmnet.com [mailto:rgrow <@t> bmnet.com]
>Sent: Thursday, December 02, 2004 11:49 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] RE: static electricity/humidity problems
>
>
>Tina,
>
>There have been several good responses regarding static. Try them. Use
>what works best in your lab.
>
>Humidity is a big problem in the southern United States. Air conditioning
>systems and de-humidifiers can't handle the load in old drafty buildings. We
>have solved this problem by adding silica gel crystals to our last absolute
>alcohol and first xylene in the dehydrating and clearing stations. This step
>has been successfully used here for many years. It also extends the life
>of the reagents 2x. We order it from: Sigma Aldrich Company, Catalog #:
>21,444.2 Silica Gel 2.5 Kg. A word of caution: Do dispense and discard
>carefully as it is a carcinogen.
>
>Good luck.
>
>Renee Grow, BA., HT (ASCP)
>rgrow <@t> bmnet.com
>Histology Supervisor
>Blount Memorial Hospital
>907 E. Lamar Alexander Pkwy.
>Maryville, TN 37804-5016
>(865) 977-4744
>(865) 977-5766 Fax
>
>From: bettina.hutz <@t> orionpharma.com [mailto:bettina.hutz <@t> orionpharma.com]
>Sent: 02 December 2004 05:26
>To: Laurie.Pereira <@t> sdcounty.ca.gov; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] static electricity[Scanned]
>
>Hello,
>
>I have the same problem during winter time - and in summer time it is the
>opposite: it is "common accepted" that in July/August there is hardly any
>staining possible, because the huminidy goes immediately to the alcohol and
>then the xylene is milky and mounting not proper. When I have been working
>in Germany for 10 years I never has this effect, but since I work in Finland
>I am fighting with it every summer. So, if anyone has a solution to the
>humidity problem, please share it with me:)
>
>Tina
>
>
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--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
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