[Histonet] Re: Type of hematoxylin stain with your bluing methods? Tell all, please.

[Gudrun Lang]

Harris - frozen sections
Mayers - HE stainer

Gudrun

----- Original Message ----- 
From: "Gayle Callis" <gcallis <@t> montana.edu>
To: "Gudrun Lang" <gu.lang <@t> gmx.at>; <Histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, December 01, 2004 4:29 PM
Subject: Type of hematoxylin stain with your bluing methods? Tell all,
please.


> Dear All,
>
> It would be appreciated that you say which hematoxylin you are using when
> you give your bluing method.  For Gudrun, are you using Mayers?
>
> At 02:59 PM 11/30/2004, you wrote:
> >Using lithiumcarbonat is the way we do it with staining frozen sections.
> >In the HE-stainer we have running tapwater. And  the sections are nice.
> >After haemalaun in special stains we let the slides in tapwater for 3-5
min.
> >
> >Gudrun Lang
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>