[Histonet] Immunostaining

LECTARD Bruno 172070 bruno.lectard <@t> cea.fr
Wed Dec 1 10:34:45 CST 2004


I have tried to get the following antibodies working, but have not had any
success. 
Rat Retinoblastoma Protein (RB) -from Santa Cruz Biotechnology, Inc. Cat #
SC-7905 and SC -102, Caveoline 1 SC-894 and  Caveolin 2 SC-1858.
I have followed suggestions on the spec sheets, tried these with and without
antigen retrieval. I would like to hear from anyone having experience with
these antibodies. If the antibody did work, please send a protocol. Also, I
am interested in knowing what other companies supply these antibodies and if
anyone has implemented these techniques. 
Thanks


Bruno Lectard 
* CEA - Fontenay aux roses 
DSV - DRR - SRCA - LCE 
BP 6 92265 Fontenay aux roses CEDEX 



<http://www-dsv.cea.fr/content/cea/d_dep/d_drr/> 





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