[Histonet] Coverslipping tape problems

Bonner, Janet Janet.Bonner <@t> FLHOSP.ORG
Tue Aug 31 12:42:04 CDT 2004

We have the same problem- and we just recut the slides as they are needed
for send out or review boards.  The important thing is to go back to glass
ASAP.  We use the automatic glass coverslippers now.
Sorry to hear about your predicament, I know it's  a rude situation to
confront.   -Janet
-----Original Message-----
From: Coulter, Diane
To: 'histonet <@t> lists.utsouthwestern.edu'
Sent: 8/31/2004 10:29 AM
Subject: [Histonet] Coverslipping tape problems

We're having serious problems with older (7-8 yrs) slides coverslipped
tape.  When these slides are retrieved form the files more often than
the tape has separated from the slide with the tissue attached to the
I've repaired some of these using Sakura's suggested method of immersing
tape in xylene for a few minutes then reattaching to the slide.  This is
messy, time consuming and usually results in air bubbles.  Any
short of recutting all these slides?

Is there any way to prevent this from happening in the first place?
now I'm ready toss the coverslipper out the window.

Thanks in advance,

Diane Coulter, RIH Histology 

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