[Histonet] Coverslipping tape problems

Coulter, Diane DCoulter <@t> Lifespan.org
Tue Aug 31 09:29:01 CDT 2004


We're having serious problems with older (7-8 yrs) slides coverslipped with
tape.  When these slides are retrieved form the files more often than not
the tape has separated from the slide with the tissue attached to the tape.
I've repaired some of these using Sakura's suggested method of immersing the
tape in xylene for a few minutes then reattaching to the slide.  This is
messy, time consuming and usually results in air bubbles.  Any suggestions,
short of recutting all these slides?

Is there any way to prevent this from happening in the first place?  Right
now I'm ready toss the coverslipper out the window.

Thanks in advance,

Diane Coulter, RIH Histology 





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