[Histonet] Trypan Blue Removal For Protein Analysis

Timothy Lewis Liparulo tliparul <@t> mix.wvu.edu
Mon Aug 30 12:48:19 CDT 2004

I am currently using Trypan Blue as a measure for injury in rat 
skeletal muscle cells.  The animals were preinjected with the dye 24 
hrs prior to sacrifice. I would like to perform a protein analysis on 
the tissue but am afraid that the dye in the tissue will be problematic 
for western blot analysis.  Does anyone know of a way to remove the dye 
pigment without significantly affecting the integrity of the muscle 

More information about the Histonet mailing list