All, I have a client that wants to do an Immunofluorescent Study on a skin punch bx. that's been in RPMI medium for 5 days. Does anyone have any thoughts as to whether or not this will work? Can anyone give me an idea on what effect the RPMI may have on the results? Thanx In Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Coordinator Milwaukee, WI