[Histonet] H&E/Alcian Blue

[Jose Luis Palazon Fernandez]

Dear all

I would like to perform an H&E/Alcian blue staining to differentiate bone and cartilage and I was wondering if I have to do the H&E first and then AB or the contrary or maybe there is a protocole to this staining technique. The acid AB solution will bleach the Hematoxylin?. If the AB is used first why not call the technique AB/H&E instead of H&E/AB?

thanks in advance

jose luis


Universidad de Oriente-Isla Margarita-Venezuela
actualmente en: Instituto de Ciencias Marinas de Andalucia
Puerto Real, Cádiz, España.
email: jluis.palazon <@t> icman.csic.es