[Histonet] fixatives

[Smith, Allen]

If you mean a fixative that is also an electron stain, potassium
permanganate (Luft,1956, J. Biophys. Biochem. Cytol. 2:799) and sodium
permanganate (Wetzel, 1961, J. Biophys. Biochem. Cytol. 9:711) have been
uses with some success.  While both are safer than osmium tetroxide, neither
gives good results as consistently.
If you just want a fixative for electron microscopy, Karnovsky's (1965)
paraformaldehyde-glutaraldehyde mixture is one of the best (J. Cell Biol.
27:137A or any EM handbook).  Plain glutaraldehyde also works: any EM
handbook will give a formula for 2.5% glutaraldehyde in pH 7.2-7.4 phosphate
or cacodylate buffer. (Note that cacodylate is very poisonous.) 

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
            Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pam plumlee
Sent: Friday, August 27, 2004 12:05 PM
To: HistoNet Server
Subject: [Histonet] Coverslipping tape



Hi all:  I know this has been asked before, is there a
quick fix for removing tissue from coverslipping tape,
reattaching to the slide, covering with a new glass
coverslip so the tissue can be photographed?  Also,
does anyone know a good alertnate fixative for osmium
tetroxide?  We are trying to keep it out of the lab. 
We have been asked to use it for a post-fix for fatty
tissue and on an EM processor.  Thanks, Pam


		
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