[Histonet] FW: Salt-split skit technique for immunofluorescence

[GUTIERREZ, JUAN]

Where I use to work, we thawed the frozen block in cold PBS and let the sections sit in it over the weekend( or at least two days) until the epithelium separated from the basement membrane.  Then pad dry carefully and refreeze.  By the way, this was all done in the refrigerator.  Good luck. 
Juan

-----Original Message-----
From: Sharon Allen [mailto:SAllen <@t> exchange.hsc.mb.ca]
Sent: Thursday, August 26, 2004 8:35 AM
To: Histonet (E-mail)
Subject: [Histonet] FW: Salt-split skit technique for immunofluorescence





> -----Original Message-----
> From:	Sharon Allen 
> Sent:	August 23, 2004 9:37 AM
> To:	Histonet (E-mail)
> Subject:	Salt-split skit technique for immunofluorescence
> 
> Hi,
> Does anyone have a method for the "Salt-split skin technique for
> immunofluorescence"?
> I would appreciate any help with this method.
> Thanks
> Sharon Allen
> sallen <@t> hsc.mb.ca
> Winnipeg, Manitoba, Canada

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