[Histonet] BOUINS AND B-5 PROCESSING

Wed Aug 25 15:23:31 CDT 2004

Tasha

We usually fixed our bone bx 's in B5 for about 2 hrs to 4hrs depending on
the size, and then went into decal. We washed the bone bx after decal and
placed it in formalin on the tissue processor. Four lymph nodes we fixed no
more than 4 hrs. The nodes were cut in half and placed in B5.

As far as Bouin's goes, the maximum time for tissue in Bouin's should be
less than 24 hrs. You must remove the picric acid out with 50% to 70%
alcohol, or I beleive you can use 70% alcohol saturated with lithium
carbonate to removed the picric acid.

I beleive Freida Carson has all of this information in her book.

Hope this helps.

Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com

                      Tasha Bourm                                                                                                                   
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                      histonet-bounces <@t> lists.utsouth        Subject:  [Histonet] BOUINS AND B-5 PROCESSING                                          
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                      08/25/2004 12:46 PM                                                                                                           

CAN ANYONE HELP ME WITH PROCESSING SOULUTIONS AND TIMES FOR BOUINS AND B-5
FIXATIVE?  I'M A STUDENT AND CAN NOT FIND THIS INFORMATION IN ANY PRINTED
MATERIAL.  THANKS FOR YOUR HELP!

TASHA BOURM
HISTOLOGY
OLYMPIC MEDICAL CENTER
PORT ANGELES, WA 98362

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