[Histonet] decal of bone marrows

[Tony Henwood]

Gayle,

What are the advantages of buffering an acid.
We use a formic acid/formalin/NaCl mix to decal after fixation in 10%
formalin/ethanol.


Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318


-----Original Message-----
From: Gayle Callis [mailto:gcallis <@t> montana.edu] 
Sent: Wednesday, 25 August 2004 12:58 AM
To: Mildred Fail; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] decal of bone marrows


Question:  Buffered formic acid, commercial preparations?  or do you make 
up an inhouse preparation?

At 07:12 AM 8/24/2004, you wrote:
>Julie,
>     We are using Formic acid, with good results on IHC.
>Rena
>
> >>> <Julie.Sanders <@t> med.va.gov> 08/24/04 08:17AM >>>

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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