[Histonet] RE: IHC polymer detection system for rat tissues

[C.M. van der Loos]

Dear Ronald,
Just use the anti-mouse EnVision for use on rat tissue specimens. However, before using it you have to mix the EnVision with 10-15% (final concentration) normal rat serum. Leave the mixture for 60 min at room temp (or overnight 4C) then use it. All the cross-reactions are abolished now.

Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  "Ronald P. Wilson" <rpw4 <@t> psu.edu> 
Date  Thu, 19 Aug 2004 15:15:43 -0400 
To  Histonet <histonet <@t> lists.utsouthwestern.edu> 
Subject  [Histonet] IHC polymer detection system for rat tissues 
Has anyone found a successful polymer detection system (i.e., Envision or
others) directed against a mouse monoclonal on rat tissue. We tried one but
got a lot of background staining, I suspect from cross reactivity with
closely related antigens. Our standard IHC approach uses an anti-mouse,
rat-absorbed secondary antibody, but I would like to get the added
sensitivity of a polymer-based system.

Ronald P. Wilson, V.M.D., M.S.
Associate Professor
Department of Comparative Medicine, HO54
Penn State University College of Medicine
M. S. Hershey Medical Center
500 University Drive
Hershey, PA 17033
phone:  717-531-8460
fax:       717-531-5001
e-mail:  rpw4 <@t> psu.edu