[Histonet] High pH AR

[Cesar Francisco Romero]

Dear Histonetters

I have had terrible results with Tris-Clorhidric.

I prefer de following buffer:

Tris/EDTA pH 9 (10/1 mM)

Buffer may be produced as follows:

1,21 g Tris, Merck 1.08387

0,37 g EDTA, Fluka Chemika 03680

1000 ml destilled water

Buffer pH is stable at 9.0 - 9.1, usually without adjustment. The buffer is 
stable for approximately 4 weeks at room temperature. I keep it in the 
icebox for several weeks. I add two drops per 1000 ml of tween 20.

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