[Histonet] IHC polymer detection system for rat tissues

[Ronald P. Wilson]

Has anyone found a successful polymer detection system (i.e., Envision or
others) directed against a mouse monoclonal on rat tissue. We tried one but
got a lot of background staining, I suspect from cross reactivity with
closely related antigens. Our standard IHC approach uses an anti-mouse,
rat-absorbed secondary antibody, but I would like to get the added
sensitivity of a polymer-based system.

Ronald P. Wilson, V.M.D., M.S.
Associate Professor
Department of Comparative Medicine, HO54
Penn State University College of Medicine
M. S. Hershey Medical Center
500 University Drive
Hershey, PA 17033
phone:  717-531-8460
fax:       717-531-5001
e-mail:  rpw4 <@t> psu.edu