[Histonet] MOM

Jackie.O'Connor <@t> abbott.com Jackie.O'Connor <@t> abbott.com
Thu Aug 19 12:55:58 CDT 2004


If your mouse tissue is from a SCID, no - (you can get some rare leaking, 
tho)
If your mouse tissue is from a nude, or immunocompetent - yes.
Does your anti-human antibody cross react with mouse?

Jackie O' 




"Dardo Ferrara" <dardofer <@t> hotmail.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/19/2004 12:39 PM

 
        To:     Histonet <@t> lists.utsouthwestern.edu
        cc: 
        Subject:        [Histonet] MOM


Hi histoneters,

I am doing immunohisto with mouse tissue and using mouse antihuman as a 
first AB. My secondary is a goat anti mouse fab fragment. With this 
combination, do I have to use a MOM blocking kit to prevent nonspecific 
binding???

Thanks

Dardo Ferrara
Cardiology Department
Emory University.
Atlanta

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