[Histonet] special stains on slides that have been to waterovernight

Bryan Hewlett bhewlett <@t> cogeco.ca
Wed Aug 18 19:46:54 CDT 2004

Hi Diana,

Provided that the tissues were NBF fixed for a minimum of 24 hours, you
should have NO problem with the common special stains and with the immuno
In fact, back at the beginning of IHC techniques this was a fairly standard
procedure with us and provided a measure of antigen retrieval for many
difficult antigens( basically it's AR at RT!).

Been there, done that!



----- Original Message -----
From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, August 18, 2004 5:56 PM
Subject: [Histonet] special stains on slides that have been to

> I was hoping I could get some advice regarding an issue.  Would a special
> stain or immuno stain on a section that has been brought to water and left
> to sit overnight in water yield reliable results.  It gets cut, dried and
> taken to water the previous day and the staining procedure is not carried
> out until the next day.  Your opinions would be appreciated.
> Diana McCaig,
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> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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