[Histonet] silver question

Bartlett, Jeanine JQB7 <@t> CDC.GOV
Tue Aug 17 19:39:31 CDT 2004

That hasn't changed:  same mountant for years.  And I need to correct something in my initial email.  The stain faded after only 2 weeks.  

	-----Original Message----- 
	From: Tony Henwood [mailto:AnthonyH <@t> chw.edu.au] 
	Sent: Tue 8/17/2004 6:25 PM 
	To: Bartlett, Jeanine; histonet <@t> lists.utsouthwestern.edu 
	Subject: RE: [Histonet] silver question

	Check the mountant you are using. Some will fade Spirochete silver stains
	very quickly
	Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
	Laboratory Manager
	The Children's Hospital at Westmead,
	Locked Bag 4001, Westmead, 2145, AUSTRALIA.
	Tel: 612 9845 3306
	Fax: 612 9845 3318
	-----Original Message-----
	From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV]
	Sent: Wednesday, 18 August 2004 2:35 AM
	To: histonet <@t> lists.utsouthwestern.edu
	Subject: [Histonet] silver question
	Has anyone experienced a problem with their Steiner's "fading" after a
	period of time?  We had a slide that had been stained with the Steiner
	procedure and it was very positive, however when it was reviewed at a
	much later date it appeared negative.  Another section was stained and
	it was positive.  I know that leaving the sections for a long time in
	xylene before they are coverslipped can lead to fading but wouldn't this
	be apparent when the slides were originally viewed? 
	Any suggestions?
	Jeanine Bartlett, HT(ASCP)
	Centers for Disease Control and Prevention
	Infectious Disease Pathology Activity
	1600 Clifton Road, MS/G-32
	Atlanta, GA 30333
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