[Histonet] silver question

Tony Henwood AnthonyH <@t> chw.edu.au
Tue Aug 17 17:25:04 CDT 2004

Check the mountant you are using. Some will fade Spirochete silver stains
very quickly


Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Bartlett, Jeanine [mailto:JQB7 <@t> CDC.GOV] 
Sent: Wednesday, 18 August 2004 2:35 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] silver question

Has anyone experienced a problem with their Steiner's "fading" after a
period of time?  We had a slide that had been stained with the Steiner
procedure and it was very positive, however when it was reviewed at a
much later date it appeared negative.  Another section was stained and
it was positive.  I know that leaving the sections for a long time in
xylene before they are coverslipped can lead to fading but wouldn't this
be apparent when the slides were originally viewed?  
Any suggestions?
Jeanine Bartlett, HT(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology Activity
1600 Clifton Road, MS/G-32
Atlanta, GA 30333
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