[Histonet] Image analysis and slide scanning machine (Wester,
Martha)
Glenn Smith
gsmith <@t> confocal.com
Mon Aug 16 15:11:45 CDT 2004
Hi Kim,
We supply such an instrument, our TISSUEscope - combining confocal scanning
laser microscopy and a wide field of view. Depending on your resolution
requirements, the TISSUEscope can acquire a single image at up to 1 um
resolution with no mosaic and no tiling of images. A standard slide (1" x
3") is loaded into the machine and the rest of operations is via software.
We have another model which offers submicron resolution as well. Both
brightfield and fluorescence detection is provided within the same
instrument. Please feel free to contact me offline for more details.
Also, we will have one of these instruments at the NSH meeting in Toronto -
we'll be exhibiting in conjunction with Beecher Instruments.
Regards
Glenn Smith, P.Eng.
519.886.9013 x38
Biomedical Photometrics Inc/GeneFocus <www.confocal.com OR
www.GeneFocus.com>
Widefield Confocal Scanning Instruments and Software
-----Original Message-----
From: Kim Merriam [mailto:kmerriam2003 <@t> yahoo.com]
Sent: Friday, August 13, 2004 9:16 AM
To: Histonet
Subject: [Histonet] Image analysis and slide scanning machine
Hello everyone,
Years ago, I heard of a machine that you could load an entire slide into,
scan the slide and then do image analysis on the tissue. I have no idea who
made this machine or even how to search for such a thing on the internet (or
the histonet archives). If anyone has the name of this company, can someone
please let me know.
I am currently doing measurements of pancreatic islets as well as
measurements of whole mouse pancreas'. I am needing to take 4-6 pictures
per pancreas in order to do this, and it is very time consuming (these
studies have up to 90 animals in them). I need a quicker and easier way to
do this!
Help!
Kim
Kim Merriam
Novartis
Cambridge, MA __________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
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------------------------------
Message: 5
Date: Fri, 13 Aug 2004 13:47:06 -0400
From: <pathrm35 <@t> adelphia.net>
Subject: RE: RE: [Histonet] a question on ethics
To: Gary Gill <garygill <@t> dcla.com>, pam marcum <mucram11 <@t> comcast.net>,
<histonet <@t> lists.utsouthwestern.edu>, Joe Nocito
<jnocito <@t> pathreflab.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<20040813174706.CQCA24693.mta13.adelphia.net <@t> mail.adelphia.net>
Content-Type: text/plain; charset=ISO-8859-1
There is also the issue that this position is not what I was told it would
be. I also didn't expect to have so many issues such as a tech that can't
work up to my standards. Also I keep asking myself if it is worth it esp.
when this isn't turning out to be the job I expected it to be. Lastly, how
can I work for someone I can't respect or trust? Any thought on that?
>
> From: Gary Gill <garygill <@t> dcla.com>
> Date: 2004/08/13 Fri PM 01:39:01 EDT
> To: "'pathrm35 <@t> adelphia.net'" <pathrm35 <@t> adelphia.net>, pam marcum
> <mucram11 <@t> comcast.net>, histonet <@t> lists.utsouthwestern.edu, Joe
Nocito
> <jnocito <@t> pathreflab.com>
> CC: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: RE: [Histonet] a question on ethics
>
> Let your physician know that the person in charge is ultimately
> responsible that the lab is run by the book. There are right ways and
> wrong ways to do things. Wrong is indefensible. Among possible
> penalties, CMS can shut down the lab. Surely your boss doesn't want
> to see the lab's name in the local newspaper headlines.
>
> Gary Gill
>
> -----Original Message-----
> From: pathrm35 <@t> adelphia.net [mailto:pathrm35 <@t> adelphia.net]
> Sent: Friday, August 13, 2004 12:34 PM
> To: pam marcum; histonet <@t> lists.utsouthwestern.edu; Joe Nocito
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: RE: [Histonet] a question on ethics
>
>
> I sent copies of incident reports to RM but my manager wants us to do
> our own internal quality control. How are things suppose to get fixed
> that way? My physician doesn't like to deal with these situations and
> refers me to my manager. I'm running in circles!!! Ron
> >
> > From: "pam marcum" <mucram11 <@t> comcast.net>
> > Date: 2004/08/13 Fri AM 09:11:34 EDT
> > To: <pathrm35 <@t> adelphia.net>, <histonet <@t> lists.utsouthwestern.edu>,
> > "Joe Nocito" <jnocito <@t> pathreflab.com>
> > Subject: RE: [Histonet] a question on ethics
> >
> >
>
------------------------------
Message: 6
Date: Fri, 13 Aug 2004 11:14:55 -0700 (PDT)
From: "A. Erickson" <andrae <@t> u.washington.edu>
Subject: RE: RE: [Histonet] a question on ethics
To: pathrm35 <@t> adelphia.net
Cc: histonet <@t> lists.utsouthwestern.edu, Gary Gill <garygill <@t> dcla.com>,
Joe Nocito <jnocito <@t> pathreflab.com>
Message-ID:
<Pine.A41.4.58.0408131113090.6754 <@t> homer05.u.washington.edu>
Content-Type: TEXT/PLAIN; charset=US-ASCII
It sounds to me, from the thread of your post(s) that you have already made
the decision to leave, just not implemented it! Good luck! Andra Erickson,
HT, Research Technologist
On Fri, 13 Aug 2004 pathrm35 <@t> adelphia.net wrote:
> There is also the issue that this position is not what I was told it
> would be. I also didn't expect to have so many issues such as a tech that
can't work up to my standards. Also I keep asking myself if it is worth it
esp. when this isn't turning out to be the job I expected it to be. Lastly,
how can I work for someone I can't respect or trust? Any thought on that?
> >
> > From: Gary Gill <garygill <@t> dcla.com>
> > Date: 2004/08/13 Fri PM 01:39:01 EDT
> > To: "'pathrm35 <@t> adelphia.net'" <pathrm35 <@t> adelphia.net>, pam marcum
> > <mucram11 <@t> comcast.net>, histonet <@t> lists.utsouthwestern.edu, Joe
Nocito
> > <jnocito <@t> pathreflab.com>
> > CC: histonet <@t> lists.utsouthwestern.edu
> > Subject: RE: RE: [Histonet] a question on ethics
> >
> > Let your physician know that the person in charge is ultimately
> > responsible that the lab is run by the book. There are right ways
> > and wrong ways to do things. Wrong is indefensible. Among possible
> > penalties, CMS can shut down the lab. Surely your boss doesn't want
> > to see the lab's name in the local newspaper headlines.
> >
> > Gary Gill
> >
> > -----Original Message-----
> > From: pathrm35 <@t> adelphia.net [mailto:pathrm35 <@t> adelphia.net]
> > Sent: Friday, August 13, 2004 12:34 PM
> > To: pam marcum; histonet <@t> lists.utsouthwestern.edu; Joe Nocito
> > Cc: histonet <@t> lists.utsouthwestern.edu
> > Subject: Re: RE: [Histonet] a question on ethics
> >
> >
> > I sent copies of incident reports to RM but my manager wants us to
> > do our own internal quality control. How are things suppose to get
> > fixed that way? My physician doesn't like to deal with these
> > situations and refers me to my manager. I'm running in circles!!!
> > Ron
> > >
> > > From: "pam marcum" <mucram11 <@t> comcast.net>
> > > Date: 2004/08/13 Fri AM 09:11:34 EDT
> > > To: <pathrm35 <@t> adelphia.net>, <histonet <@t> lists.utsouthwestern.edu>,
> > > "Joe Nocito" <jnocito <@t> pathreflab.com>
> > > Subject: RE: [Histonet] a question on ethics
> > >
> > >
> >
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 7
Date: Fri, 13 Aug 2004 14:15:19 -0400
From: "Linda Blazek" <BlazekL <@t> childrensdayton.org>
Subject: RE: RE: [Histonet] a question on ethics
To: pathrm35 <@t> adelphia.net, mucram11 <@t> comcast.net, garygill <@t> dcla.com,
jnocito <@t> pathreflab.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <s11ccd20.041 <@t> NW-GWIA2>
Content-Type: text/plain; charset=US-ASCII
Seems to me you have already shut down and have made up your mind that you
are done and don't want to accept some of the great ideas that have been
offered today. If your goal is to solve the problems you have encountered
then the histonet has offered some wonderful help.
>>> <pathrm35 <@t> adelphia.net> 8/13/2004 1:47:06 PM >>>
There is also the issue that this position is not what I was told it would
be. I also didn't expect to have so many issues such as a tech that can't
work up to my standards. Also I keep asking myself if it is worth it esp.
when this isn't turning out to be the job I expected it to be. Lastly, how
can I work for someone I can't respect or trust? Any thought on that?
>
> From: Gary Gill <garygill <@t> dcla.com>
> Date: 2004/08/13 Fri PM 01:39:01 EDT
> To: "'pathrm35 <@t> adelphia.net'" <pathrm35 <@t> adelphia.net>, pam marcum
> <mucram11 <@t> comcast.net>, histonet <@t> lists.utsouthwestern.edu, Joe
Nocito
> <jnocito <@t> pathreflab.com>
> CC: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: RE: [Histonet] a question on ethics
>
> Let your physician know that the person in charge is ultimately
responsible
> that the lab is run by the book. There are right ways and wrong ways
to do
> things. Wrong is indefensible. Among possible penalties, CMS can
shut down
> the lab. Surely your boss doesn't want to see the lab's name in the
local
> newspaper headlines.
>
> Gary Gill
>
> -----Original Message-----
> From: pathrm35 <@t> adelphia.net [mailto:pathrm35 <@t> adelphia.net]
> Sent: Friday, August 13, 2004 12:34 PM
> To: pam marcum; histonet <@t> lists.utsouthwestern.edu; Joe Nocito
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: RE: [Histonet] a question on ethics
>
>
> I sent copies of incident reports to RM but my manager wants us to do
our
> own internal quality control. How are things suppose to get fixed
that way?
> My physician doesn't like to deal with these situations and refers me
to my
> manager. I'm running in circles!!! Ron
> >
> > From: "pam marcum" <mucram11 <@t> comcast.net>
> > Date: 2004/08/13 Fri AM 09:11:34 EDT
> > To: <pathrm35 <@t> adelphia.net>, <histonet <@t> lists.utsouthwestern.edu>,
> > "Joe Nocito" <jnocito <@t> pathreflab.com>
> > Subject: RE: [Histonet] a question on ethics
> >
> >
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Fri, 13 Aug 2004 14:35:09 -0400
From: <pathrm35 <@t> adelphia.net>
Subject: RE: RE: [Histonet] a question on ethics
To: "Linda Blazek" <BlazekL <@t> childrensdayton.org>,
<mucram11 <@t> comcast.net>, <garygill <@t> dcla.com>,
<jnocito <@t> pathreflab.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<20040813183509.TGEP9204.mta10.adelphia.net <@t> mail.adelphia.net>
Content-Type: text/plain; charset=ISO-8859-1
I am willing to accept any and all ideas for a resolution. I value everyones
opinion on histonet, that's why I posted my situation.
>
> From: "Linda Blazek" <BlazekL <@t> childrensdayton.org>
> Date: 2004/08/13 Fri PM 02:15:19 EDT
> To: pathrm35 <@t> adelphia.net, mucram11 <@t> comcast.net, garygill <@t> dcla.com,
> jnocito <@t> pathreflab.com
> CC: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: RE: [Histonet] a question on ethics
>
> Seems to me you have already shut down and have made up your mind that
> you are done and don't want to accept some of the great ideas that
> have been offered today. If your goal is to solve the problems you
> have encountered then the histonet has offered some wonderful help.
>
>
> >>> <pathrm35 <@t> adelphia.net> 8/13/2004 1:47:06 PM >>>
>
> There is also the issue that this position is not what I was told it
> would be. I also didn't expect to have so many issues such as a tech
> that can't work up to my standards. Also I keep asking myself if it is
> worth it esp. when this isn't turning out to be the job I expected it
> to be. Lastly, how can I work for someone I can't respect or trust?
> Any thought on that?
> >
> > From: Gary Gill <garygill <@t> dcla.com>
> > Date: 2004/08/13 Fri PM 01:39:01 EDT
> > To: "'pathrm35 <@t> adelphia.net'" <pathrm35 <@t> adelphia.net>, pam marcum
> > <mucram11 <@t> comcast.net>, histonet <@t> lists.utsouthwestern.edu, Joe
> Nocito
> > <jnocito <@t> pathreflab.com>
> > CC: histonet <@t> lists.utsouthwestern.edu
> > Subject: RE: RE: [Histonet] a question on ethics
> >
> > Let your physician know that the person in charge is ultimately
> responsible
> > that the lab is run by the book. There are right ways and wrong
> > ways
> to do
> > things. Wrong is indefensible. Among possible penalties, CMS can
> shut down
> > the lab. Surely your boss doesn't want to see the lab's name in the
> local
> > newspaper headlines.
> >
> > Gary Gill
> >
> > -----Original Message-----
> > From: pathrm35 <@t> adelphia.net [mailto:pathrm35 <@t> adelphia.net]
> > Sent: Friday, August 13, 2004 12:34 PM
> > To: pam marcum; histonet <@t> lists.utsouthwestern.edu; Joe Nocito
> > Cc: histonet <@t> lists.utsouthwestern.edu
> > Subject: Re: RE: [Histonet] a question on ethics
> >
> >
> > I sent copies of incident reports to RM but my manager wants us to
> > do
> our
> > own internal quality control. How are things suppose to get fixed
> that way?
> > My physician doesn't like to deal with these situations and refers
> > me
> to my
> > manager. I'm running in circles!!! Ron
> > >
> > > From: "pam marcum" <mucram11 <@t> comcast.net>
> > > Date: 2004/08/13 Fri AM 09:11:34 EDT
> > > To: <pathrm35 <@t> adelphia.net>, <histonet <@t> lists.utsouthwestern.edu>,
>
> > > "Joe Nocito" <jnocito <@t> pathreflab.com>
> > > Subject: RE: [Histonet] a question on ethics
> > >
> > >
> >
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
------------------------------
Message: 9
Date: Fri, 13 Aug 2004 15:05:21 -0400
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: [Histonet] Diastase dilemma
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s11cd8c1.081 <@t> GHSGWIANW2.GEISINGER.EDU>
Content-Type: text/plain; charset=US-ASCII
Bizarre things happen here all the time, but this is REALLY BIZARRE! We'll
do a PAS w/ diastase and our control tissue will be digested but the patient
tissue won't be. They are mounted on the same slide!
The next day we will run the stain again and we tried turning the slide
upside-down in the Coplin jar. Same problem. We mixed fresh diastase
solution and tried again. Same problem. Tomorrow, we may run it again and it
will work fine. What could be going on? I'm perplexed!
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.
------------------------------
Message: 10
Date: Fri, 13 Aug 2004 14:27:53 -0500
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
Subject: RE: [Histonet] Diastase dilemma
To: "Angela Bitting" <akbitting <@t> geisinger.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <JFEMICGBHEGPLAMIJPJPEEPDCFAA.JNocito <@t> Pathreflab.com>
Content-Type: text/plain; charset="US-ASCII"
Angela,
It could be that the patient section contains mucin, which will not digest
out. If this is not the case, then I suggest trying to digest the slides on
a slide rack or something to lay the slides on a horizontal surface. Just my
21/2 cents worth.
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Angela
Bitting
Sent: Friday, August 13, 2004 2:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Diastase dilemma
Bizarre things happen here all the time, but this is REALLY BIZARRE! We'll
do a PAS w/ diastase and our control tissue will be digested but the patient
tissue won't be. They are mounted on the same slide! The next day we will
run the stain again and we tried turning the slide upside-down in the Coplin
jar. Same problem. We mixed fresh diastase solution and tried again. Same
problem. Tomorrow, we may run it again and it will work fine. What could be
going on? I'm perplexed!
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Fri, 13 Aug 2004 14:31:07 -0500
From: Jackie.O'Connor <@t> abbott.com
Subject: Re: [Histonet] Diastase dilemma
To: "Angela Bitting" <akbitting <@t> geisinger.edu>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFBE4F851C.89AACDFB-ON86256EEF.006B033F <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
Did you stop to think that your patient just might be positive for
glycogen?
"Angela Bitting" <akbitting <@t> geisinger.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/13/2004 02:05 PM
To: <histonet <@t> lists.utsouthwestern.edu>
cc:
Subject: [Histonet] Diastase dilemma
Bizarre things happen here all the time, but this is REALLY BIZARRE! We'll
do a PAS w/ diastase and our control tissue will be digested but the
patient tissue won't be. They are mounted on the same slide!
The next day we will run the stain again and we tried turning the slide
upside-down in the Coplin jar. Same problem.
We mixed fresh diastase solution and tried again. Same problem. Tomorrow, we
may run it again and it will work fine. What could be going on? I'm
perplexed!
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It
is intended solely for the addressee. Access to this message by anyone
else is unauthorized. If you are not the intended recipient, any
disclosure, copying, distribution or any action taken, or omitted to be
taken, in reliance on it is prohibited and may be unlawful. If you have
received this message in error, please delete all electronic copies of
this message (and the documents attached to it, if any), destroy any hard
copies you may have created and notify me immediately by replying to this
email. Thank you. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Fri, 13 Aug 2004 13:44:05 -0600
From: "Daryl Mikita" <dmikita <@t> wmcnet.org>
Subject: [Histonet] pHing of eosin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s11cc5b2.012 <@t> gwgate.wmcnet.org>
Content-Type: text/plain; charset=US-ASCII
Hello,
We are looking at pHing our eosin to the correct pH, instead of just using
it straight from the bottle. What should we use to adjust the pH? Should
it be 1N sodium hydroxide or 1N hydrochloric acid?
Thanks
Daryl
------------------------------
Message: 13
Date: Fri, 13 Aug 2004 13:47:19 -0600
From: "Daryl Mikita" <dmikita <@t> wmcnet.org>
Subject: [Histonet] Another question
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s11cc669.013 <@t> gwgate.wmcnet.org>
Content-Type: text/plain; charset=US-ASCII
Hello,
We were looking at our HQIP and we were wondering where in the H&E staining
procedure, people use heat? We could not think of any place except for
drying the slides.
Thanks
Daryl
------------------------------
Message: 14
Date: Fri, 13 Aug 2004 15:07:51 -0500
From: Gary Gill <garygill <@t> dcla.com>
Subject: RE: [Histonet] pHing of eosin
To: 'Daryl Mikita' <dmikita <@t> wmcnet.org>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <0B11620AEE5EFB4FA7A7558339972A8307048A <@t> HALIBUT.dcla.com>
Content-Type: text/plain
Suggest using glacial acetic acid: 5 mL per liter of eosin. Be prepared for
a color explosion.
Gary Gill
-----Original Message-----
From: Daryl Mikita [mailto:dmikita <@t> wmcnet.org]
Sent: Friday, August 13, 2004 2:44 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] pHing of eosin
Hello,
We are looking at pHing our eosin to the correct pH, instead of just using
it straight from the bottle. What should we use to adjust the pH? Should
it be 1N sodium hydroxide or 1N hydrochloric acid?
Thanks
Daryl
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Fri, 13 Aug 2004 16:11:47 -0400
From: Amos Brooks <amosbrooks <@t> earthlink.net>
Subject: [Histonet] a question on ethics
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <6.0.0.22.0.20040813154852.01be9008 <@t> pop.earthlink.net>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Ron,
When a ship is hitting a reef there is 2 options. First option:
Bail out, hit the life rafts and get out of there before you go down with
the ship. Second option is to get the ship off the reef and repair the
damage. This sounds like a problematic situation and you may be in a good
position to make drastic improvements to the system. The question you need
to decide is which course you intend to take, and stick with it.
If you decide to leave, do it cautiously as these problems could
still be pinned on you as the guy that came in caused problems and left. (I
know that's not the way it is but people often shift blame for problems on
people that can't defend themselves). You still should mention in an exit
interview (assuming you get one) about the issues, so that someone else can
fix them when you are gone, and so it is documented that you were aware of
the situation and not the cause.
If you decide to fix the ship, expect some rough waters. Very
often when you point out errors to people they start looking for things to
complain about with you. You should proceed with caution and document,
document, document! The root cause survey that was previously mentioned
sounds like a good plan. Often people make errors that they can prevent
just by a simple modification of their procedure. Establishing redundancy
is a good way to prevent most of the errors you have described. For
example, picking a block and a slide up and comparing them before they go
into the microtome, then again after the slide is cut and finally comparing
the block to the slides before sending them out. Time consuming, yes, but
it is hard to make the same mistake 3 times. Similar redundancies can be
established in most other situations too. Turn around time will probably
suffer a bit, but if it keeps you from getting sued it's worth it. Good luck
which ever way you choose, Amos Brooks
At 09:29 AM 8/13/2004, you wrote:
>Message: 9
>Date: Fri, 13 Aug 2004 04:27:14 -0500
>From: "Ron Martin" <pathrm35 <@t> adelphia.net>
>Subject: [Histonet] a question on ethics
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <002701c48117$b8cbc750$5dedeb44 <@t> Pathrm35>
>Content-Type: text/plain; charset="iso-8859-1"
>
>Fellow techs,
>I am in a difficult situation and need some serious advice. I recently
>(4
>months ago) accepted a technical supervisor position in a dermatology lab.
>I went from a bench tech at my old job to this position. I also walked
>away from a raise at my old position so I could step into a supervision
>position. I took the new position because I was told by my manager that
>she would teach me some supervision, management and financial skills that
>I currently do not have as well as the growth potential of the
>company.This position is not what I was told it would be. Part of my
>"duties" include emptying the trash, clean the bathroom (not happening)
>and taking her personal and professional calls. The question on ethics is
>the high volume of mistakes made by our technician, our offices and also
>by my manager herself.
>I caught a mistake made by our tech a few weeks ago.She put the wrong
>tissue on the wrong slides as she inverted the two cases. I caught her
>mistake before it went out. One time she assigned the same number to two
>different cases. She then sent the correct case out for a consult (which
>wasn't needed) and put the wrong patient name on the slide. Every other
>day there is something different.My manager will not terminate her as she
>knows I am seeking employment elsewhere and she cannot afford to lose a
tech.
>One day my manager gave three cases the same accession number.She caught
>her mistake on one of the cases but I still ended up with two cases with
>the same number. When I first started there my manager had a case in which
>there " was no tissue in the container". She said that she notified the
>office about the situation however up to six weeks later the office was
>still calling looking for results.At that point she wrote up an incident
>report and dated it six weeks prior to coincide with the surgery date.
>Our offices are not any better. One case came in with the wrong patient
>information. It took about 7 weeks for the office to realize that they
>sent the wrong patient information with the biopsy as the names were close
>in spelling. The offices continually send mistakes with incorrect
>spelling of patients names and incorrect anatomical locations.
> I have tired to document everything but there are too many mistakes and
> I don't have enough time or energy to keep up with them.My manager wants
> us to do our own "internal quality control". My interpretation of this is
> that she doesn't want our physician and risk manager to know of these
> mistakes. Are these becoming common problems or is it just my situation?
>I want to emphasize that I hope I am not being unethical myself for
>revealing this information but I really need some advice and support.I
>have very high standards and they are not being met in this current
>situation. I am currently seeking a new position but I need employment and
>cannot resign until another position becomes available. I would like to
>stay in Florida and if anyone knows of any positions please inform me as I
>am at my wits end. Thanks in advance.
>
>Ron
------------------------------
Message: 16
Date: Fri, 13 Aug 2004 15:12:37 -0500
From: "GUTIERREZ, JUAN" <juan.gutierrez <@t> christushealth.org>
Subject: RE: [Histonet] You may want to hit the delete button with
this, then again, maybe not
To: "Joe Nocito" <JNocito <@t> Pathreflab.com>, "Histonet"
<histonet <@t> pathology.swmed.edu>
Message-ID:
<A61F23E937E4DA488E0C0F60093843D90F6A54 <@t> ccetxm030.echristus.net>
Content-Type: text/plain; charset="iso-8859-1"
Hey at least they tried to talk to you(a year later)but they tried YOU!
Like I told you on my last e-mail my behind is about two pounds lighter
after my boss bit some of it off because of my comments on the histonet
about a certain German company who should remain nameless. Apparently my
speaking the truth, the whole truth, and nothing but the truth on this forum
made them a little mad. If they made good products to begin with, we
wouldn't be having this conversation. Have anybody noticed how many pages
of the phonebook are dedicated to lawyers? There is about twice the number
of lawyers compared to doctors. Aint that scary? I wonder when lawyer
season is going to open, heck there's more of them than there is deer. Just
kidding. I know one good lawyer, one.
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533
My views are my own and do not reflect those of my employer. Long live free
speech! "We have nothing to fear but fear itself".
-----Original Message-----
From: Joe Nocito [mailto:JNocito <@t> Pathreflab.com]
Sent: Thursday, August 12, 2004 3:35 PM
To: Histonet
Subject: [Histonet] You may want to hit the delete button with this,then
again, maybe not
Afternoon Netter's,
Remember about a week or two the topic was getting "flamed"? Well,
here's a new one. Sometime last year, I made several postings about a
certain company and their product that I used and it did not work for me. I
wrote them a letter and returned all the merchandise back to them saying I
was not happy with their product. I waited several months and never got a
response, so I named the company on the Histonet. Since then, I have been
throwing away their flyers, catalogs and any other correspondence I receive.
Obviously, they are doing fine without my business and I am doing fine
without their products.
Today, I receive a letter from the company with a copy of my
Histonet postings from over a year ago, telling me that this has been in
customer service for about a year (hmmmmmmmm, I'm trying to find out where
the customer service is in all of this. (One year, okay, I'll get back to
that), that their customer service has tried several times to contact me but
to no avail. (Hmmmmm, one way would have been to respond to my email
address, but I'll come back to this also).
So after all this time, they want to do business with me. (So close
to the NSH meeting, hmmmmmmmmm, another point to ponder).
My big beef is that these companies read this forum and try to use
strong arm tactics on people who post negative comments about them or their
products. I have many friends that are sales reps in this field (I'm talking
like come over my house and eat type of friends) and I love these people.
I must reiterate that this forum was set up to exchange ideas,
theories and experiences, both positive and negative. If this was such a
"hot item", point #1, why did it take a whole year to get a response when
they had my company and address on file? Is this how customer service works?
I don't think so!!
Point #2 If you are a vendor and don't like a particular posting,
why don't you reply back to that person via email.
Point #3 don't wait until it's almost time for the NSH annual S/C to
fix a problem, it's probably too late by then.
Point #4 If you haven't had a purchase order or have not
corresponded with a customer for over a year, chances are that customer does
not want to do business with you. Let lying dogs lie and don't open old
scars. All you did was get me going on a tangent. I know, there goes Nocito
again, but I refuse to be treated like this from any one. Like they say in
the movies, "Don't call me, I'll call you".
Okay, I've said my piece now let the FLAMING BEGIN!!!!!!!!!
As always, the opinions stated by this author in no way reflects the
opinions of his employer, subordinates, friends or relatives.
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Fri, 13 Aug 2004 15:12:07 -0500
From: Jackie.O'Connor <@t> abbott.com
Subject: Re: [Histonet] Diastase dilemma
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF7EC0959D.47A5DCF3-ON86256EEF.006ECA9A <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
Once I read my own answer, I saw how stupid it is. My apologies. It's
been a long week. Think before you type, Jack . . .think.....
Jackie.O'Connor <@t> abbott.com
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/13/2004 02:31 PM
To: "Angela Bitting" <akbitting <@t> geisinger.edu>
cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Diastase dilemma
Did you stop to think that your patient just might be positive for
glycogen?
"Angela Bitting" <akbitting <@t> geisinger.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/13/2004 02:05 PM
To: <histonet <@t> lists.utsouthwestern.edu>
cc:
Subject: [Histonet] Diastase dilemma
Bizarre things happen here all the time, but this is REALLY BIZARRE! We'll
do a PAS w/ diastase and our control tissue will be digested but the
patient tissue won't be. They are mounted on the same slide!
The next day we will run the stain again and we tried turning the slide
upside-down in the Coplin jar. Same problem.
We mixed fresh diastase solution and tried again. Same problem. Tomorrow, we
may run it again and it will work fine. What could be going on? I'm
perplexed!
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It
is intended solely for the addressee. Access to this message by anyone
else is unauthorized. If you are not the intended recipient, any
disclosure, copying, distribution or any action taken, or omitted to be
taken, in reliance on it is prohibited and may be unlawful. If you have
received this message in error, please delete all electronic copies of
this message (and the documents attached to it, if any), destroy any hard
copies you may have created and notify me immediately by replying to this
email. Thank you. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Fri, 13 Aug 2004 14:15:00 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] pHing of eosin
To: "Daryl Mikita" <dmikita <@t> wmcnet.org>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20040813141110.01b227a8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
If you are buying eosin Y, already made up, check the MSDS, and the pH, it
may be adjusted correctly. I know that this is the case for Richard Allan
Eosin Y solution. If you are making up eosin y from the dry dye, then the
recipe is (Hrapchak and Sheehan)
eosin y 0.5g
95% ethanol 50 ml
glacial acetic acid 1 drop
If you are adjusting already made up, use acetic acid.
At 01:44 PM 8/13/2004, you wrote:
>Hello,
>
>We are looking at pHing our eosin to the correct pH, instead of just
>using
>it straight from the bottle. What should we use to adjust the pH? Should
>it be 1N sodium hydroxide or 1N hydrochloric acid?
>
>Thanks
>Daryl
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 19
Date: Fri, 13 Aug 2004 16:15:48 -0400
From: "TERRI BRAUD" <terribraud <@t> msn.com>
Subject: [Histonet] RE: a Question of Ethics
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY11-F6kkOO1Spm7js000051fd <@t> hotmail.com>
Content-Type: text/plain; format=flowed
I can certainly understand the frustration of working in new management
position that does not offer the training and responsibilities to go with
it. And while I support going over a manager's head when patient care is
impacted, you also must ask yourself if the activity is being tolerated by
the pathologist(s)? If so, then look for another job, because you MUST have
the support of your medical staff. Beyond that, I would encourage you to
look for management training from outside sources, such as the program
offered at the NSH convention, on-line courses in Quality Improvement and
Laboratory Management, subscription to the Manager's edition of Advance,
etc. These will help give you the educational tools to get started, but
they in no way will make you an effective leader. Speaking from 15 years of
management experience, to be an effective leader you have to be out in
front, not someone who just points the way. As for any job, this often means
"paying dues" by performing "other" duties until you are accepted as part of
the team. If this means you have to clean bathrooms or empty waste to show
you are part of a team, then do it cheerfully for a time. If a team sees
your willingness to perform the "nasty" tasks, then eventually they will be
more willing to take on those tasks, and others, to free your time for
management duties. Anyone can point to problems within any system, but the
true leader is the one that offers concrete solutions and suggestions and is
willing to go the extra miles to put them into place. As we all know,
Histology is a manual labor intensive process, prone to identity errors. As
a leader, you must provide your employees with a system that has enough
system checks and double checks that limit their ability to make a mistake,
or catch it before it impacts patient care. Then, when mistakes are made,
use a carefully constructed system to document and to review the system
process. Maybe these "mistake-prone" employees have suffered the same
frustrations that you have, even to the point of not caring anymore. Have
they been given the tools, the training, the education needed to perform
their jobs accurately and with positive feedback? If you, as a new manager,
are not being helped, how much do you think is trickling down to their
level? I would guess very little. This lab may or may not be the place to
learn to be a supervisor, but it sounds like a place that is "ripe" for the
picking. Anyone can step into a smooth running lab, but having the
opportunity to make a real difference in a poorly functioning lab is not as
easy. Laboratory administrators often come from a clinical background with
very little AP experience and usually welcome the Histology Supervisor that
comes in with fresh ideas to improve a lab's performance. Study process
improvement. Offer well thought out, budget neutral suggestions for process
improvement. And remember, being a supervisor means that your most important
skill should be in "people management". This means coming up with ways to
inspire and empower your employees to do a better job! Good luck with your
quest, where ever it takes you.
Sincerely,
Terri L. Braud, HT(ASCP)
Surgical Pathology Manager
University of Virginia Health Systems
Charlottesville, VA 22908
------------------------------
Message: 20
Date: Fri, 13 Aug 2004 15:30:12 -0500
From: "Stapf, Ross" <RossS <@t> BaylorHealth.edu>
Subject: RE: [Histonet] Diastase dilemma
To: "Angela Bitting" <akbitting <@t> geisinger.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C4FA30B7EA78EF46A3B3BC8869AC1F771393F9 <@t> BHDAEXCH11.bhcs.pvt>
Content-Type: text/plain; charset="US-ASCII"
Are you running a plain PAS on the patient as well? I was always taught to
always run a PAS and PASD even if the Dr only wanted a PASD. It serves as a
control.
If you are doing that, and know there is no diference digested vs undigested
in the patient then I don't know what to say. I would normally look to
something in the tissue that is causing this.
If you were still using spit to digest I would blame it on you eating sugar
before spitting on the slide, or not drinking enough coffee.
Ross M Stapf
Histopathology Manager
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
214-820-2465
214-820-4110 fax
RossS <@t> baylorhealth.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Friday, August 13, 2004 2:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Diastase dilemma
Bizarre things happen here all the time, but this is REALLY BIZARRE! We'll
do a PAS w/ diastase and our control tissue will be digested but the patient
tissue won't be. They are mounted on the same slide! The next day we will
run the stain again and we tried turning the slide upside-down in the Coplin
jar. Same problem. We mixed fresh diastase solution and tried again. Same
problem. Tomorrow, we may run it again and it will work fine. What could be
going on? I'm perplexed!
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This e-mail, facsimile, or letter and any files or attachments transmitted
with it contains information that is confidential and privileged. This
information is intended only for the use of the individual(s) and
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further disclosures are prohibited without proper authorization. If you are
not the intended recipient, any disclosure, copying, printing, or use of
this information is strictly prohibited and possibly a violation of federal
or state law and regulations. If you have received this information in
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or via e-mail at privacy <@t> baylorhealth.edu. Baylor Health Care System, its
subsidiaries, and affiliates hereby claim all applicable privileges related
to this information.
------------------------------
Message: 21
Date: Fri, 13 Aug 2004 15:46:36 -0500
From: "Linda Davis" <lindas <@t> awesomenet.net>
Subject: [Histonet] Re: Smears on the Ventana
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <007c01c48176$bfbe2490$05a16243 <@t> D6JLZ851>
Content-Type: text/plain; charset="iso-8859-1"
Terre,
We fix the smear in 95% alcohol, rinse in water, and place on the Ventana to
run along with the paraffin sections.
Linda Davis, HT (ASCP)
Histology Supervisor
Rio Grande Regional Hospital
McAllen, TX.
------------------------------
Message: 22
Date: Fri, 13 Aug 2004 16:29:01 -0500
From: "Charles.Embrey" <Charles.Embrey <@t> carle.com>
Subject: RE: [Histonet] Diastase dilemma
To: "'Jackie.O'Connor <@t> abbott.com'" <Jackie.O'Connor <@t> abbott.com>
Cc: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E0DA23E35D80D611B80600A0C9EA33D5018AB6BE <@t> exchange1.carle.com>
Content-Type: text/plain
Did you ever stop to think.... that if the patient tissue were positive for
Glycogen then the digestion would remove it and it would show no PAS
staining? The positive reaction on could be mucin or acid mucosubstances.
Charles Embrey
-----Original Message-----
From: Jackie.O'Connor <@t> abbott.com [mailto:Jackie.O'Connor <@t> abbott.com]
Sent: Friday, August 13, 2004 2:31 PM
To: Angela Bitting
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Diastase dilemma
Did you stop to think that your patient just might be positive for
glycogen?
"Angela Bitting" <akbitting <@t> geisinger.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/13/2004 02:05 PM
To: <histonet <@t> lists.utsouthwestern.edu>
cc:
Subject: [Histonet] Diastase dilemma
Bizarre things happen here all the time, but this is REALLY BIZARRE! We'll
do a PAS w/ diastase and our control tissue will be digested but the
patient tissue won't be. They are mounted on the same slide!
The next day we will run the stain again and we tried turning the slide
upside-down in the Coplin jar. Same problem.
We mixed fresh diastase solution and tried again. Same problem. Tomorrow, we
may run it again and it will work fine. What could be going on? I'm
perplexed!
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It
is intended solely for the addressee. Access to this message by anyone
else is unauthorized. If you are not the intended recipient, any
disclosure, copying, distribution or any action taken, or omitted to be
taken, in reliance on it is prohibited and may be unlawful. If you have
received this message in error, please delete all electronic copies of
this message (and the documents attached to it, if any), destroy any hard
copies you may have created and notify me immediately by replying to this
email. Thank you. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 23
Date: Fri, 13 Aug 2004 14:35:07 -0700 (PDT)
From: "Y. Wang" <ynwang <@t> u.washington.edu>
Subject: [Histonet] MMP-1 immuno labelling of placenta
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<Pine.A41.4.58.0408131428450.204322 <@t> homer09.u.washington.edu>
Content-Type: TEXT/PLAIN; charset=US-ASCII
Hello,
Has anyone had any experience immuno labelling placenta tissue with
anti-MMP1? We have tried this (cryosections with ABC kit with DAB) but are
getting alot of (what we assume to be) background and nothing that looks
like images we have seen on-line. Seeing as we have not had any experience
with placenta tissue we are questioning if we are took the tissue from the
correct place from the placenta (we were given a whole one which we took
random samples from).
Thank you for your help
Yak-Nam Wang
Senior Fellow
Department of Bioengineering
University of Washington
Box 357962
Seattle, WA 98195
Tel.: (206)-221-5873
Fax.: (206)-221-5874
------------------------------
Message: 24
Date: Fri, 13 Aug 2004 16:40:44 -0500
From: "Kapoor, Sue" <Sue.Kapoor <@t> uhsi.org>
Subject: RE: [Histonet] RE: a Question of Ethics
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<61E9F2400F53D5119CFC00508B44E33B019F563D <@t> khmcexch.uhsi.org>
Content-Type: text/plain; charset="iso-8859-1"
As for any job, this often means
"paying dues" by performing "other" duties until you are accepted as part of
the team. If this means you have to clean bathrooms or empty waste to show
you are part of a team, then do it cheerfully for a time.
-I'm sorry, but as a Histology Supervisor, cleaning bathrooms or emptying
waste does NOT show you are a "part of the team"...it shows that management
has no respect for your position. Why isn't the housekeeping dept. doing
these duties?
Sue Kapoor, HT (ASCP)
Histology Coordinator
Kenosha Medical Center
Kenosha, WI
262-653-5570
------------------------------
Message: 25
Date: Fri, 13 Aug 2004 18:03:58 -0400
From: Diana McCaig <dmccaig <@t> ckha.on.ca>
Subject: [Histonet] acid washed glassware
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <3E5A3F039F0BD8118B4700C00D002024043250 <@t> CKHA9>
Content-Type: text/plain
What concentration and what acid do you use to clean glassware prior to
silver impregnation staining. Do you simply rinse it or is it soaked for a
specific period of time? Air or oven dried? Diana McCaig, R.T.
Charge Tech, Histology
Chatham Kent Health Alliance
519-352-6401 (6604)
------------------------------
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End of Histonet Digest, Vol 9, Issue 23
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