[Histonet] Substitute fixative for B5

[GUTIERREZ, JUAN]

Hi Linda, we are using B-Plus and doing our immunos on the Ventana system (Benchmarks).  So far no problems with any of the common lymphocyte markers.  We didn't even have to change our protocols.  Good luck.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My views are my own and do not reflect those of my employer.
Long live free speech!  "We have nothing to fear but fear itself".


-----Original Message-----
From: Prestridge, Linda [mailto:LPrestridge <@t> seton.org]
Sent: Monday, August 16, 2004 11:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Substitute fixative for B5


We are trying to quit using B5.
We are did study with B-Plus from BBc Chemical, IBF from Surgipath,
Alcoholic Zinc from ANatech and AZF from Newcomers.
 
Well the pathologists ruled it down to B-Plus and AZF from Newcomers.
We then did battery of immuno's - Lymphoma panel. Most stained fair, and
they narrowed it down to B-Plus.
But...the problem is that I can't get the kappa and lambda (poly's) to
stain. I tried AR and got some staining but very dirty.
 
What are any of you using and have you had the same problem getting the
kappa and lambda to stain???
 
Thanks, Linda
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