[Histonet] Substitute fixative for B5

[Prestridge, Linda]

We are trying to quit using B5.
We are did study with B-Plus from BBc Chemical, IBF from Surgipath,
Alcoholic Zinc from ANatech and AZF from Newcomers.
 
Well the pathologists ruled it down to B-Plus and AZF from Newcomers.
We then did battery of immuno's - Lymphoma panel. Most stained fair, and
they narrowed it down to B-Plus.
But...the problem is that I can't get the kappa and lambda (poly's) to
stain. I tried AR and got some staining but very dirty.
 
What are any of you using and have you had the same problem getting the
kappa and lambda to stain???
 
Thanks, Linda