[Histonet] Diastase dilemma

[Jackie.O'Connor <@t> abbott.com]

Once I read my own answer, I saw how stupid it is.  My apologies.  It's 
been a long week.  Think before you type, Jack . . .think.....




Jackie.O'Connor <@t> abbott.com
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/13/2004 02:31 PM

 
        To:     "Angela Bitting" <akbitting <@t> geisinger.edu>
        cc:     histonet <@t> lists.utsouthwestern.edu, 
histonet-bounces <@t> lists.utsouthwestern.edu
        Subject:        Re: [Histonet] Diastase dilemma


Did you stop to think that your patient just might be positive for 
glycogen?






"Angela Bitting" <akbitting <@t> geisinger.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/13/2004 02:05 PM

 
        To:     <histonet <@t> lists.utsouthwestern.edu>
        cc: 
        Subject:        [Histonet] Diastase dilemma


Bizarre things happen here all the time, but this is REALLY BIZARRE!
We'll do a PAS w/ diastase and our control tissue will be digested but the 

patient tissue won't be. They are mounted on the same slide! 
The next day we will run the stain again and we tried turning the slide 
upside-down in the Coplin jar. Same problem.
We mixed fresh diastase solution and tried again. Same problem.
Tomorrow, we may run it again and it will work fine.
What could be going on?
I'm perplexed!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916


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