[Histonet] blistering skin condotions

[Crick Tony]

Hi Sarah

We use a direct method, with FITC conjugated IgG, IgA, IgM etc. It is a
simples yet quick and effective method with results available within 1hr.


Tony Crick
Morecambe Bay Hospitals

-----Original Message-----
From: Blachford, Sarah - Histopath Main
[mailto:Sarah.Blachford <@t> ngh.nhs.uk]
Sent: 10 August 2004 12:55
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] blistering skin condotions



Dear All, 
	I am in the process of setting up a reliable technique(s) to
demonstrate blistering skin conditions.  The gold standard is to use
flourescence.  What method do people use, and do you use conjugated FITC
antibodies or separate antibodies and fluorochromes.  Is any body using
tTgase as part of their panel along with the normal IG's, C3

Thanks




Northampton General Hospital NHS Trust 
Cliftonville, Northampton NN1 5BD 

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