[Histonet] RE: Glass vs Tape coverslippers
Aldo Anile
aldo.anile <@t> HDScientific.com.au
Mon Aug 9 21:21:27 CDT 2004
There is little question that if all things being equal, glass would be the preferred option for coverslipping. Some glass coverslippers today have addressed the main advantages that tape has had over glass, speed and wet slides.
The Medite RCM7000 (Meisei Promounter) has the ability to coverslip at a rate of 450 slides/hr. It also has a slide drying fan that produces touch dry slides, eliminating the messy wet slide issue that has historically been associated with glass coverslipping.
Anyone that has gone back to slides years after being coverslipped with tape would be familiar with the deteriorating nature of tape coverslipping over time.
Aldo Anile
Applications Consultant
HD SCIENTIFIC SUPPLIES PTY LTD (Aust)
E-mail: aldo.anile <@t> hdscientific.com.au
Web: www.hdscientific.com.au
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, 6 August 2004 23:29
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 9, Issue 8
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Today's Topics:
1. RE: Glass vs. Tape Coverslippers (Weems, Joyce)
2. Re: Staining for Macrophages (Sharon Cooperman)
3. Re: Private responses - well said, Barry! (Gayle Callis)
4. Re: Private responses - well said, Barry! (Susan Q Wells)
5. RE: Private responses (Mass Histology Service)
6. (no subject) (Browning Deb)
7. Recall: (Browning Deb)
8. retrieval puzzle, forgot this line on last message (Browning Deb)
9. Jeff Lowen (James Watson)
10. Benchmark XT (Lab)
11. BRDU free floating vibratome sections (Kimberle M. Jacobs)
12. ID-2 (Kimberle M. Jacobs)
13. RE: Benchmark XT (Joe Nocito)
14. Re: BRDU free floating vibratome sections (Sarah Jones)
15. H. pylori control tissue (Richard Cartun)
16. RE: Private responses (Tony Henwood)
17. RE: electrical charge slides (Bill Sinai)
18. Rank and Rank L (Patricia Bourne)
19. Problem with Masson's Trichrome (Bdeer24 <@t> aol.com)
20. Re: H. pylori control tissue (lpwenk <@t> sbcglobal.net)
21. Fixative for FACS (Myri37 <@t> aol.com)
22. Oncocytoma vs. Chromophobe (Barnhart, Tammy)
23. Re: Problem with Masson's Trichrome (rschoon)
24. Cytochrome oxidase (COX) staining: non-carcinogenic
alternative to DAB? (Martin Hasselblatt)
25. Masson Trichrome (Hermina Borgerink)
----------------------------------------------------------------------
Message: 1
Date: Thu, 5 Aug 2004 13:09:25 -0400
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] Glass vs. Tape Coverslippers
To: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>, "Histonet
\(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<83AACDB0810528418AA106F9AE9B7F7E0A626B <@t> sjhaexc02.sjha.org>
Content-Type: text/plain; charset="iso-8859-1"
I was demoing coverslippers and didn't tell the pathologists. Thought I'd see if they could tell the difference. One of our pathologists made us remove the tape from her entire days workload and recoverslip them by hand. Oh well.....
Joyce
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Morken,
Tim - Labvision
Sent: Thursday, August 05, 2004 12:08 PM
To: 'Histonet (E-mail)'
Subject: RE: [Histonet] Glass vs. Tape Coverslippers
Gary, We did find that plastic was no good for cytology specimens - at least
the lumpy ones - it wouldn't form a flat surface. For thin-prep it worked
great. I think the quality is OK for routine histology. In our lab we did
testing in the lab and when satisfied it was working well we switched over
without telling the pathologists. About two weeks later the Chief
Pathologist asked me when we would start using the plastic coverslips!
Tim Morken
-----Original Message-----
From: Gary Gill [mailto:garygill <@t> dcla.com]
Sent: Thursday, August 05, 2004 8:53 AM
To: 'Laurie Colbert'; WWmn916 <@t> aol.com; Histonet (E-mail)
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Glass vs. Tape Coverslippers
Plastic is no substitute for glass. Just because one doesn't "see" a
difference doesn't mean there isn't a difference. Whether the difference
makes a real difference in outcomes is another story.
ASTM specs for cover glasses apply to glass, not to tape. The higher the
numerical aperture of an objective, and the better the objective quality
(i.e., achromat, fluorite, apochromat -- plan and non-plan), the more likely
that one will see imaging differences when tape is used. Of course, using
glass doesn't ensure good quality.
Practical stuff like mounting medium and cover glass thickness, clean
lenses, and Kohler illumination also play a real role. Image quality can't
be better than the weakest link.
The specs are relative to the impact of the physical and optical properties
of glass on light as it passes through the mounting medium and glass through
the objective. For this reason, it's not nice to fool Mother Nature and use
plastic.
Gary Gill
-----Original Message-----
From: Laurie Colbert [mailto:laurie.colbert <@t> huntingtonhospital.com]
Sent: Thursday, August 05, 2004 10:23 AM
To: WWmn916 <@t> aol.com; Histonet (E-mail)
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Glass vs. Tape Coverslippers
We have a tape coverslipper, and we love it. It is fast and we have had
very few problems with it. Our pathologists have no problem reading the
slides, and as far as I know, there's never been a problem photographing a
slide. We did demo the glass coverslippers when we were first looking for a
new coverslipper, and there were too many problems with slides sticking
together, air bubbles, and it was just all-around not as "user friendly."
Laurie Colbert
Huntington Hospital
-----Original Message-----
From: WWmn916 <@t> aol.com [mailto:WWmn916 <@t> aol.com]
Sent: Wednesday, August 04, 2004 8:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Glass vs. Tape Coverslippers
Hello again,
I'm looking for opinions on the subject of glass coverslippers versus tape
coverslipping. I have the opportunity to decide on a system. My only
experience has been with tape coverslipping. I understand machines that
glass
coverslip are slower than tape systems. Is the refractive index better with
glass
coverslips under the microscope? Opinions pros/cons are appreciated.
Deb King, HT(ASCP)
Sacramento, CA
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------------------------------
Message: 2
Date: Thu, 5 Aug 2004 13:24:18 -0400
From: Sharon Cooperman <scoop <@t> mail.nih.gov>
Subject: Re: [Histonet] Staining for Macrophages
To: Gayle Callis <gcallis <@t> montana.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <p0602042cbd381d667d22@[156.40.160.118]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"
Thanks, Gail, you're right. Does FA-11 work on FFPE tissue and do
you need HIER? Serotec doesn't say.
Thanks,
Sharon
>Sorry, but ED-1 (CD68) is a Mouse anti-Rat antibody for a subset of
>rat macrophages. It may cross react to mouse but you would be doing
>doing mouse on mouse IHC. The CD68 for mouse is rat anti-mouse,
>clone FA-11 for murine peritoneal macrophages. SEROTEC is a source
>of these antibodies, including the F4/80 (Rat anti-mouse).
>
>A sidenote: SEROTEC has a free macrophage differentiation poster
>available upon request.
>
>At 06:58 PM 8/4/2004, you wrote:
>
>>You can use ED-1 from Serotec. It works on FFPE tissue with pH 6.0
>>citrate antigen retrieval and on frozen tissue without AR. You can
>>also use F4/80 on frozen tissue. Both are rat monoclonals and you
>>can use an anti-rat secondary Ab cross adsorbed against mouse IgG.
>>You could also also use ricin 1 from Vector or E-Y Laboratories
>>which binds mouse macrophages. Ricin 1 is not the dangerous ricin.
>>I don't know details of how to use it (I did once, but I don't
>>remember). There are books available or the manufacturers may know
>>or maybe some other Histonet folks. I have used ED1 and F4/80 on
>>mouse tissues and I think it's your best bet.
>>
>>Sharon
>>
>>>I had previously asked about MAC-387 staining. Unfortunately, we
>>>can't use the staining because we are staining mouse kidney and it
>>>doesn't look right if you stain with a mouse anti-body. Does
>>>anyone know of a way to stain for macrophages with out an
>>>antibody??? Or perhaps a polyclonal antibody???? Thanks a bunch!
>>>
>>>
>>>Jennifer K. Sipes, RALAT
>>>Sr. Laboratory Technician
>>>Johns Hopkins University
>>>Ross 929
>>>720 Rutland Avenue
>>>Baltimore, MD 21205
>>>phone: 410-614-0131
>>>cell: 443-413-0853
>>>e-mail: jengirl1014 <@t> yahoo.com
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>>---------------------------------
>>>Do you Yahoo!?
>>>Yahoo! Mail - 50x more storage than other providers!
>>>_______________________________________________
>>>Histonet mailing list
>>>Histonet <@t> lists.utsouthwestern.edu
>>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>
>>
>>--
>>Sharon Cooperman <scoop <@t> mail.nih.gov>
>>NIH, NICHD, CBMB 301.435-7735
>>Building 18T, room 101 301.402-0078 fax
>>Bethesda, MD 20892
>>
>>_______________________________________________
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)
--
Sharon Cooperman <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB 301.435-7735
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892
------------------------------
Message: 3
Date: Thu, 05 Aug 2004 11:41:52 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] Private responses - well said, Barry!
To: mucram11 <@t> comcast.net, Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20040805112541.01afad70 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"
Pam and all,
Too many people forget or ignore the fact that some individuals who
are experts in the field of histotechnology are not employed in just
clinical or research laboratories. If one is involved with a company
doing technical research and development of a product destined for
histoland, then they are should be very welcome to comment.
Knowledge should not be smothered (flames!) I don't see you as a
salesperson, but a valuable resource providing me with information I
can choose to use or not. All part of the learning experience.
Complaints = delete button.
At 10:13 AM 8/5/2004, you wrote:
I agree with both Gayle and Barry yet have one reservation. I was
hit several times on this server for answering very general
questions and told salespeople should not be on HistoNet. Although
my answers had nothing to do with any product we sell and by the
way I am not a sales person, just technical and product
development. After the second incident that several others came on
and agreed we were not welcome I stay off 99% of the time. It is
difficult to walk the line here and not be on privately only. I do
agree that if it is a sales pitch it should not be on the line as a
reply that should be private answers only.
Thanks for letting me say this and I hope no one is offended.
Pam Marcum
-------------- Original message --------------
> Thank you Barry - very well said.
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> Bozeman MT 59717-3610
>
> At 09:02 AM 8/5/2004, you wrote:
>
> >I hate to see the word "flamed" as here in sunny Houston it
has been in
> >the upper 90s for about 2 weeks.
> >
> >While I appreciate people's perception about getting
"flamed" when they
> >have posted items, I have a real problem with posting most
items
> >privately.
> >Histonet is an extremely valuable resource and there is a
vast amount of
> >expertise available. It is only logical for this knowledge
and
> >experience to be f! reely shared.
> >I can't say that I have been "flamed" (or even mildly
scorched) but
> >sometimes I make mistakes and post incorrect information. It
is a
> >valuable learning experience for me and all interested
parties to have
> >any errors corrected. Sometimes it is matter of perception
and how
> >criticism is leveled (being brutally frank is not a good
attitude).
> >"Flaming" may be perceived by one individual to have
occurred while
> >another may regard a response as constructive criticism.
> >If anyone here gets "flamed" or has their employer contacted
by a
> >company about a posting then please let us know. Companies
do respond
> >positively to public opinion. Any companies with such an
attitude this
> >should be held accountable and so should employers for not
telling the
> >company or their representative to take a hike.
> >I do believe t! hat if someone requests private responses
that this be
>!
; >re spected. Second, I believe that when responding one
should be careful
> >when criticizing a piece of equipment or a company. Negative
criticism
> >sticks and while this may be true for a particular
individual it could
> >be an isolated incident and due to many factors. Not
everyone knows
> >how to deal effectively with companies (and not every
company knows how
> >to deal responsibly with customers). I think that such
listings should
> >be private. The individual who receives these could then let
us know
> >about the final results in the form of a summary.
> >If I am to get "flamed" for this or any other postings
please include
> >"flamed" at the start of the message so that I don't' just
think that
> >the response is constructive criticism.
> >Barry
> >
> >
> >_______________________________________________
> >Histonet mailing list > >Histonet <@t> lists.utsouthwestern.edu
> >[1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
References
1. http://lists.utsouthwestern.edu/mailman/listinfo/histonet
2. http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Thu, 05 Aug 2004 14:20:13 -0400
From: Susan Q Wells <susan.wells <@t> bms.com>
Subject: Re: [Histonet] Private responses - well said, Barry!
To: Gayle Callis <gcallis <@t> montana.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <41127A5D.4080406 <@t> bms.com>
Content-Type: text/plain; format=flowed; charset=us-ascii
I completely agree with Gayle! This list is invaluable to most of us
working in the field.
Gayle Callis wrote:
> Pam and all,
> Too many people forget or ignore the fact that some individuals who
> are experts in the field of histotechnology are not employed in just
> clinical or research laboratories. If one is involved with a company
> doing technical research and development of a product destined for
> histoland, then they are should be very welcome to comment.
> Knowledge should not be smothered (flames!) I don't see you as a
> salesperson, but a valuable resource providing me with information I
> can choose to use or not. All part of the learning experience.
> Complaints = delete button.
>
>
> At 10:13 AM 8/5/2004, you wrote:
>
> I agree with both Gayle and Barry yet have one reservation. I was
> hit several times on this server for answering very general
> questions and told salespeople should not be on HistoNet. Although
> my answers had nothing to do with any product we sell and by the
> way I am not a sales person, just technical and product
> development. After the second incident that several others came on
> and agreed we were not welcome I stay off 99% of the time. It is
> difficult to walk the line here and not be on privately only. I do
> agree that if it is a sales pitch it should not be on the line as a
> reply that should be private answers only.
> Thanks for letting me say this and I hope no one is offended.
> Pam Marcum
>
> -------------- Original message --------------
> > Thank you Barry - very well said.
> >
> > Gayle Callis
> > MT,HT,HTL(ASCP)
> > Research Histopathology Supervisor
> > Veterinary Molecular Biology
> > Montana State University - Bozeman
> > Bozeman MT 59717-3610
> >
> > At 09:02 AM 8/5/2004, you wrote:
> >
> > >I hate to see the word "flamed" as here in sunny Houston it
> has been in
> > >the upper 90s for about 2 weeks.
> > >
> > >While I appreciate people's perception about getting
> "flamed" when they
> > >have posted items, I have a real problem with posting most
> items
> > >privately.
> > >Histonet is an extremely valuable resource and there is a
> vast amount of
> > >expertise available. It is only logical for this knowledge
> and
> > >experience to be f! reely shared.
> > >I can't say that I have been "flamed" (or even mildly
> scorched) but
> > >sometimes I make mistakes and post incorrect information. It
> is a
> > >valuable learning experience for me and all interested
> parties to have
> > >any errors corrected. Sometimes it is matter of perception
> and how
> > >criticism is leveled (being brutally frank is not a good
> attitude).
> > >"Flaming" may be perceived by one individual to have
> occurred while
> > >another may regard a response as constructive criticism.
> > >If anyone here gets "flamed" or has their employer contacted
> by a
> > >company about a posting then please let us know. Companies
> do respond
> > >positively to public opinion. Any companies with such an
> attitude this
> > >should be held accountable and so should employers for not
> telling the
> > >company or their representative to take a hike.
> > >I do believe t! hat if someone requests private responses
> that this be
> >!
> ; >re spected. Second, I believe that when responding one
> should be careful
> > >when criticizing a piece of equipment or a company. Negative
> criticism
> > >sticks and while this may be true for a particular
> individual it could
> > >be an isolated incident and due to many factors. Not
> everyone knows
> > >how to deal effectively with companies (and not every
> company knows how
> > >to deal responsibly with customers). I think that such
> listings should
> > >be private. The individual who receives these could then let
> us know
> > >about the final results in the form of a summary.
> > >If I am to get "flamed" for this or any other postings
> please include
> > >"flamed" at the start of the message so that I don't' just
> think that
> > >the response is constructive criticism.
> > >Barry
> > >
> > >
> > >_______________________________________________
> > >Histonet mailing list > >Histonet <@t> lists.utsouthwestern.edu
> > >[1]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Gayle Callis
>
> MT,HT,HTL(ASCP)
>
> Research Histopathology Supervisor
>
> Veterinary Molecular Biology
>
> Montana State University - Bozeman
>
> PO Box 173610
>
> Bozeman MT 59717-3610
>
> 406 994-6367 (lab with voice mail)
>
> 406 994-4303 (FAX)
>
>References
>
> 1. http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 2. http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 5
Date: Thu, 5 Aug 2004 14:33:59 -0400
From: "Mass Histology Service" <jstaruk <@t> masshistology.com>
Subject: RE: [Histonet] Private responses
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <MBBBIJCAJFLPINLKIACDMEFECLAA.jstaruk <@t> masshistology.com>
Content-Type: text/plain; charset="iso-8859-1"
Especially the archives! Quite often a topic is of no interest to me at the
present time yet a week later, I have to stain for that certain CD antibody
that I remember was discussed in detail the prior week.
I use the archives several times a week and if all responses were private,
there would be no archives!
Jim
____________________
James Staruk, HT(ASCP)
Mass Histology Service
www.masshistology.com
------------------------------
Message: 6
Date: Thu, 5 Aug 2004 15:05:35 -0400
From: Browning Deb <browning <@t> HHSC.CA>
Subject: [Histonet] (no subject)
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3AADFB88753AD31189C100902786B91C0E27836D <@t> hch_nt_exchange.hhsc.ca>
Content-Type: text/plain; charset="iso-8859-1"
Here is a puzzle. In order to streamline the IHC workload, I have had the
techs organize the slides in vertical racks that can be used for dewaxing,
heat retrieval, and quenching (meth/px), instead of the old way of dewaxing
in one set of racks, switching to other racks for retrieval, and then into
coplin jars for quenching. Sounds great. We ended up with an unusual
vertical staining pattern with central staining of the tissues, but both
edges that corresponded with the edges of the slide racks had no staining.
The slides were heated at 60 deg. overnight. Slides that did not require
heat retrieval were not affected, therefore it was not a dewaxing problem.
All slides lay flat for application of antibodies and detection system. I
know of another site that vertically handles their slides, and they do not
get this artefact, the only difference between them and us is quenching
before retrieval vs after. Any suggestions? We went back to the old way,
and the artefact is gone, nothing changed except the position (vertical vs
horizontal) of the slides. Looking forward to scientific explanations,
inquiring minds want to know.
Debra Browning, ART
Technical Specialist, Immunohistochemistry
Anatomic Pathology
Hamilton Health Sciences
phone: (905) 527-4322 ext 46131
e-mail: browning <@t> hhsc.ca
fax: (905) 524-2681
This information is directed in confidence solely to the person named above
and may not otherwise be distributed, copied or disclosed. Therefore, this
information should be considered strictly confidential. If you have
received this email in error, please notify the sender immediately via a
return email for further direction. Thank you for your assistance.
------------------------------
Message: 7
Date: Thu, 5 Aug 2004 15:07:12 -0400
From: Browning Deb <browning <@t> HHSC.CA>
Subject: [Histonet] Recall:
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3AADFB88753AD31189C100902786B91C0E27836E <@t> hch_nt_exchange.hhsc.ca>
Content-Type: text/plain
Browning Deb would like to recall the message, "".
This information is directed in confidence solely to the person named above
and may not otherwise be distributed, copied or disclosed. Therefore, this
information should be considered strictly confidential. If you have
received this email in error, please notify the sender immediately via a
return email for further direction. Thank you for your assistance.
------------------------------
Message: 8
Date: Thu, 5 Aug 2004 15:07:41 -0400
From: Browning Deb <browning <@t> HHSC.CA>
Subject: [Histonet] retrieval puzzle, forgot this line on last message
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3AADFB88753AD31189C100902786B91C0E27836F <@t> hch_nt_exchange.hhsc.ca>
Content-Type: text/plain; charset="iso-8859-1"
Here is a puzzle. In order to streamline the IHC workload, I have had the
techs organize the slides in vertical racks that can be used for dewaxing,
heat retrieval, and quenching (meth/px), instead of the old way of dewaxing
in one set of racks, switching to other racks for retrieval, and then into
coplin jars for quenching. Sounds great. We ended up with an unusual
vertical staining pattern with central staining of the tissues, but both
edges that corresponded with the edges of the slide racks had no staining.
The slides were heated at 60 deg. overnight. Slides that did not require
heat retrieval were not affected, therefore it was not a dewaxing problem.
All slides lay flat for application of antibodies and detection system. I
know of another site that vertically handles their slides, and they do not
get this artefact, the only difference between them and us is quenching
before retrieval vs after. Any suggestions? We went back to the old way,
and the artefact is gone, nothing changed except the position (vertical vs
horizontal) of the slides. Looking forward to scientific explanations,
inquiring minds want to know.
Debra Browning, ART
Technical Specialist, Immunohistochemistry
Anatomic Pathology
Hamilton Health Sciences
phone: (905) 527-4322 ext 46131
e-mail: browning <@t> hhsc.ca
fax: (905) 524-2681
This information is directed in confidence solely to the person named above
and may not otherwise be distributed, copied or disclosed. Therefore, this
information should be considered strictly confidential. If you have
received this email in error, please notify the sender immediately via a
return email for further direction. Thank you for your assistance.
------------------------------
Message: 9
Date: Thu, 5 Aug 2004 12:31:21 -0700
From: "James Watson" <jwatson <@t> gnf.org>
Subject: [Histonet] Jeff Lowen
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7BA50F61CB491B4692B8BCFBB656B5F903428986 <@t> EXCHCLUSTER01.lj.gnf.org>
Content-Type: text/plain; charset="us-ascii"
Jeff Lowen,
Please contact me. Thank you.
James Watson HT, ASCP
Facilities Manager of Histology
GNF, C015
858-332-4647
jwatson <@t> gnf.org
------------------------------
Message: 10
Date: Thu, 5 Aug 2004 14:50:20 -0400
From: "Lab" <lab <@t> mcpathology.com>
Subject: [Histonet] Benchmark XT
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001101c47b1d$0f0f5e70$70cfa8c0 <@t> LAB>
Content-Type: text/plain; charset="iso-8859-1"
Our lab has had 2 ventana nexes systems and we are looking to upgrade to 2 benchmark xt units.We have had one in our lab to demo for approx. 3 months.During this time we have had 2 thermal pad issues and several file corruptions. Has anyone with a benchmark xt had similar problems and if so how often? Any opions about the instrument would be helpful.
Thanks,
Amy Boan,HT
------------------------------
Message: 11
Date: Thu, 05 Aug 2004 16:32:43 -0400
From: "Kimberle M. Jacobs" <kmjacobs <@t> vcu.edu>
Subject: [Histonet] BRDU free floating vibratome sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5.1.0.14.2.20040805162931.01c9f2a8 <@t> mail1.vcu.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Hi I have searched the archives and found a lot of discussion of BRDU, but
no answer to the question if there is a protocol out there for
formaldehyde-fixed, vibratome-cut, free floating sections. Actually we
could mount them on slides as well, I just don't want to do the whole
paraffin embedding thing.
Also is it really necessary to have thin sections if you are going to use
pepsin and acid, etc. ??
We could probably cut as thin as 20 um, but would prefer to use 40 or 60 um.
We will be doing this in pregnant ferrets (E36, gestation is ~42 days) and
examining the brains at ages P0-P15.
Thanks very much for any help!!
Kimberle M. Jacobs, Ph.D.
Assistant Professor
Department of Anatomy and Neurobiology
Virginia Commonwealth University
P.O. Box 980709
Richmond, VA 23298-0709
(804) 827-2135
FAX: (804) 828-9477
kmjacobs <@t> vcu.edu
------------------------------
Message: 12
Date: Thu, 05 Aug 2004 16:36:57 -0400
From: "Kimberle M. Jacobs" <kmjacobs <@t> vcu.edu>
Subject: [Histonet] ID-2
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5.1.0.14.2.20040805163247.01c9d160 <@t> mail1.vcu.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Has anyone used the antibody to ID-2? We purchased it from Santa Cruz
Biotech. They had some recommendations for western blot, but none for
immunohistochemistry.
Our goal is simply to label the superficial layers of ferret visual
cortex. But on our first try using 1:50, 1:100, and 1:200 primary
dilutions - it didn't work at all.
We were wondering if we need to do some antigen revealing step. Or perhaps
it will not be found this caudal?
Anyone know of any antibodies that label a specific layer in visual
cortex? I've heard that the otx1 antibody is not working well right
now. We are going to get RORbeta to attempt to stain layer IV, but again -
no specific protocol for it.
Thanks very much for any help!
Kimberle M. Jacobs, Ph.D.
Assistant Professor
Department of Anatomy and Neurobiology
Virginia Commonwealth University
P.O. Box 980709
Richmond, VA 23298-0709
(804) 827-2135
FAX: (804) 828-9477
kmjacobs <@t> vcu.edu
------------------------------
Message: 13
Date: Thu, 5 Aug 2004 15:44:15 -0500
From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
Subject: RE: [Histonet] Benchmark XT
To: "Lab" <lab <@t> mcpathology.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <JFEMICGBHEGPLAMIJPJPKEMFCFAA.JNocito <@t> Pathreflab.com>
Content-Type: text/plain; charset="iso-8859-1"
Amy,
I have a new carousal waiting to be installed as we speak. I have 2 XT's,
the oldest one since February of this year. I've had 8 different heating
pads go bad on three different occasions since February. Most of the time,
we place our control on the same slide as the patient, so if the control
worked, there is no question that everything worked okay. If the control is
negative, then we repeat the slide on the newer machine.
In all fairness to Vetnana, they have responded well to our phone calls and
emails. I think this is an issue that they are trying to work on in house.
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Lab
Sent: Thursday, August 05, 2004 1:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Benchmark XT
Our lab has had 2 ventana nexes systems and we are looking to upgrade to 2
benchmark xt units.We have had one in our lab to demo for approx. 3
months.During this time we have had 2 thermal pad issues and several file
corruptions. Has anyone with a benchmark xt had similar problems and if so
how often? Any opions about the instrument would be helpful.
Thanks,
Amy Boan,HT
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Thu, 05 Aug 2004 16:57:22 -0500
From: "Sarah Jones" <sjones <@t> cvm.tamu.edu>
Subject: Re: [Histonet] BRDU free floating vibratome sections
To: <histonet <@t> lists.utsouthwestern.edu>,<kmjacobs <@t> vcu.edu>
Message-ID: <s112670e.030 <@t> cvm.tamu.edu>
Content-Type: text/plain; charset=US-ASCII
Hi Kimberle,
I've done quite a bit of vibratome sectioning and free floating
staining. It's quite challenging! Two ways I can think of that might
help you. One would be to use the Shandon coverplate system. The
sections would be floated onto slides and then the coverplate carefully
placed on to the slide. It then fits into a holder and it gravity feeds
the reagents over the slide. It uses a very small amount of reagent.
The other way is to handle the free floating sections in netwells or you
can make your own using open cassettes and small petri dishes. The most
important steps would be making sure the rinsing steps were sufficient.
The netwell/petri dish method would use a much greater amount of
reagent. Hope this helps. Sarah
Sarah Jones, HT(ASCP)
Histology Lab
Dept. of Veterinary Integrative Biosciences
College of Veterinary Medicine
Texas A&M University
College Station, TX 77843-4458
phone: 979-845-3177
fax: 979-458-3499
>>> "Kimberle M. Jacobs" <kmjacobs <@t> vcu.edu> 08/05/04 3:32 PM >>>
Hi I have searched the archives and found a lot of discussion of BRDU,
but
no answer to the question if there is a protocol out there for
formaldehyde-fixed, vibratome-cut, free floating sections. Actually we
could mount them on slides as well, I just don't want to do the whole
paraffin embedding thing.
Also is it really necessary to have thin sections if you are going to
use
pepsin and acid, etc. ??
We could probably cut as thin as 20 um, but would prefer to use 40 or 60
um.
We will be doing this in pregnant ferrets (E36, gestation is ~42 days)
and
examining the brains at ages P0-P15.
Thanks very much for any help!!
Kimberle M. Jacobs, Ph.D.
Assistant Professor
Department of Anatomy and Neurobiology
Virginia Commonwealth University
P.O. Box 980709
Richmond, VA 23298-0709
(804) 827-2135
FAX: (804) 828-9477
kmjacobs <@t> vcu.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 05 Aug 2004 19:39:09 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] H. pylori control tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s1128ce9.071 <@t> gwmail.harthosp.org>
Content-Type: text/plain; charset=US-ASCII
Is anyone looking for H. pylori control tissue? We just had a partial
resection of stomach and it is loaded with bugs. If there is an
overwhelming response, I may just send control blocks to the NSH tissue
bank for distribution.
Richard
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Hartford Hospital
Hartford, CT 06102
(860) 545-1596
------------------------------
Message: 16
Date: Fri, 6 Aug 2004 12:31:42 +1000
From: Tony Henwood <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Private responses
To: "'Dawson, Glen'" <GDawson <@t> Milw.Dynacare.com>
Cc: 'HistoNet Server' <histonet <@t> pathology.swmed.edu>
Message-ID: <1CF2E2E5BB36D5119E7A0008C791F3740800E230 <@t> simba.kids>
Content-Type: text/plain
Glen,
I respect your right to free choice and accept your views.
Keep well,
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: Dawson, Glen [mailto:GDawson <@t> Milw.Dynacare.com]
Sent: Friday, 6 August 2004 12:03 AM
Cc: 'HistoNet Server'
Subject: [Histonet] Private responses
Baowei & All,
If you don't want to deal with large amounts of grief, I would encourage you
to respond privately. Due to threats of calls to my employer, lawsuits,
profanity, constant flaming of every response I post and attaining my own
little histonet stalker (you know who you are), I keep 99% of all my
responses private. There are a number of people & vendors on this
listserver that revel in tearing down the posts of others rather than
creating helpful or insightful posts themselves. It is not a bad thing for
you to post privately to avoid these problems.
I actually used to post publicly up to the point that I was dealing with
personal attacks more than I was gleaning useful information from the
histonet. Engaging in those types of interactions was too time consuming,
draining & enfuriating. Expressing one's own view is nice and, yes, it
should be OK, but it isn't. I see no value in "shaming" someone into
posting publicly. If you don't want to...don't.
The funny thing is that I'll be getting flamed for this post which is
whining about being flamed...isn't it ironic?
Just My OPINION,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Coordinator
Milwaukee, WI
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------------------------------
Message: 17
Date: Fri, 6 Aug 2004 12:54:41 +1000
From: "Bill Sinai" <bills <@t> icpmr.wsahs.nsw.gov.au>
Subject: RE: [Histonet] electrical charge slides
To: <DDittus787 <@t> aol.com>, "histonet \(E-mail\)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000201c47b60$b8b82810$e1ce080a <@t> wsahs.nsw.gov.au>
Content-Type: text/plain; charset="iso-8859-1"
Dana,
There was heaps written about this on Histonet previously, look in the
archives.
We found that the problem was associated with the Plus slides when used in
conjunction with an EDTA buffer as the material used to coat the slides is a
calcium salt and the EDTA was affecting the coating by chelating the
calcium!!!
All the best
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
DDittus787 <@t> aol.com
Sent: Friday, 06 August 2004 12:10 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] electrical charge slides
To my colleagues:
I have a question.Is anyone out there having occasional staining(orlack of
staining on IHc slides?) I was told that the charge or the way the slides
are charged can have (pardon the pun) a negative effect. Does anyone know
/experience or have the scientific data for this?
Dana
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------------------------------
Message: 18
Date: Thu, 5 Aug 2004 19:55:56 -0700 (PDT)
From: Patricia Bourne <p_bourne_14526 <@t> yahoo.com>
Subject: [Histonet] Rank and Rank L
To: HistoNet Server <histonet <@t> pathology.swmed.edu>
Message-ID: <20040806025556.77653.qmail <@t> web53706.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hello everyone....Has anyone worked successfully with Rank and Rank L? I've been through three (3) antibody sets and still not great results.....Help is really needed on this one. Thanks in advance for the help and information.
---------------------------------
Do you Yahoo!?
Yahoo! Mail Address AutoComplete - You start. We finish.
------------------------------
Message: 19
Date: Fri, 06 Aug 2004 03:19:37 -0400
From: Bdeer24 <@t> aol.com
Subject: [Histonet] Problem with Masson's Trichrome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <10A0ACD8.74118519.0015B53C <@t> aol.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Everyone!
I was recommended to try the Masson's Trichrome stainings on the slides of uterine tissue that I have been collecting (Previously I had only performed Hematoxylin & Eosin stains). This is a new method to our lab and thus far, staining has not gone well. Although my sections contain a significant amount of muscle, I have only been able to see the aniline blue stain. On a couple of the slides, there was a slight dark purple coloration on the epithelial lining.
I have noticed upon comparing the protocol we received to others found on the internet that ours lacks the step in which the slides are placed in Bouin's fluid. There was also no specification in our protocol for the type of hematoxylin, so we had been using the same hematoxylin (Harris's modified with acetic acid) that we use in our H & E stainings. I was wondering if any of these might have led to the stainings not going as planned. The tissue sections also looked slightly brittle, perhaps due to a harsher stain. I am wondering if this has anything to do with my fixation process in which I use a 4% paraformaldehyde/PBS mixture, ethanol and xylene steps, as well as embedding the tissues in paraffin.
Is Bouin's a crucial step, would I have better staining with a different tissue fixation, or should I be looking in a different direction? Any suggestions would be very much appreciated!
Thanks!
Amber Greenbank
Arizona State University
------------------------------
Message: 20
Date: Fri, 6 Aug 2004 04:44:36 -0400
From: <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] H. pylori control tissue
To: "Richard Cartun" <Rcartun <@t> harthosp.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <007d01c47b91$9b6e7bc0$7434d445 <@t> domainnotset.invalid>
Content-Type: text/plain; charset="iso-8859-1"
I believe how the NSH Control bank works is that you let NSH control block
coordinator know what you are willing to share. You then hang onto the
blocks, and if anyone contacts NSH asking for a specific control, they
receive a list of the people willing to share. That person then calls
someone on the list, asks for the control, and the person with the control
sends it out.
Below is the NSH control bank person, if I remember correctly.
Quality Control
Ethel Macrea
Ventana Medical Systems
1910 E. Innovation Park Drive
Tucson, AZ 85739
800-227-2155 ext. 3856
emacrea <@t> ventanamed.com
Hope this helps.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI
----- Original Message -----
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, August 05, 2004 7:39 PM
Subject: [Histonet] H. pylori control tissue
> Is anyone looking for H. pylori control tissue? We just had a partial
> resection of stomach and it is loaded with bugs. If there is an
> overwhelming response, I may just send control blocks to the NSH tissue
> bank for distribution.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Hartford Hospital
> Hartford, CT 06102
> (860) 545-1596
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Fri, 06 Aug 2004 05:34:20 -0400
From: Myri37 <@t> aol.com
Subject: [Histonet] Fixative for FACS
To: histonet <@t> pathology.swmed.edu
Message-ID: <69986EA4.0E45DDBE.0005167B <@t> aol.com>
Content-Type: text/plain; charset=iso-8859-1
hello
Do you know which fixative can i use for intracellular immunostaining for FACS ?
We use saponine 0.1 % for permeabilisation.
Thank you for your help
Myriam
Natural implant
------------------------------
Message: 22
Date: Fri, 6 Aug 2004 06:11:22 -0500
From: "Barnhart, Tammy" <Tbarnhart <@t> primecare.org>
Subject: [Histonet] Oncocytoma vs. Chromophobe
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1779904B5E82D511914C00D0B793339205BFD830 <@t> exchangent>
Content-Type: text/plain; charset="iso-8859-1"
I was wondering if there is(are) any immunohistochemical antibodies or
special stains besides Hale's Colloidal Iron for the differentiation of
Oncocytoma and Chromophobe in kidney? I am not able to make the dialysised
(sp?) iron needed for the Hale's but would like to offer my pathologist an
alternate. Any suggestions?
Tammy Barnhart, BS, HTL(ASCP)
Anatomic Pathology Supervisor
St. Alexius Medical Center
Bismarck, ND
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------------------------------
Message: 23
Date: Fri, 06 Aug 2004 08:25:17 -0400
From: rschoon <rschoon <@t> email.unc.edu>
Subject: Re: [Histonet] Problem with Masson's Trichrome
To: Bdeer24 <@t> aol.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <411378AD.8020806 <@t> email.unc.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
Amber,
Don't know where you got your protocol but I would suggest ANY of the standerd Histology books. As for an answer to your problems.
1- mordent in either Boin's fluid or saturated picric acid for 60 min. at 60oC (there are other time, temp.'s and concentrations if one looks them up). The method simply will not work without the modent.
2- use Weigert's Iron Hematoxylin as the nuclear stain.
Robert Schoonhoven
Bdeer24 <@t> aol.com wrote:
>Hi Everyone!
>
>I was recommended to try the Masson's Trichrome stainings on the slides of uterine tissue that I have been collecting (Previously I had only performed Hematoxylin & Eosin stains). This is a new method to our lab and thus far, staining has not gone well. Although my sections contain a significant amount of muscle, I have only been able to see the aniline blue stain. On a couple of the slides, there was a slight dark purple coloration on the epithelial lining.
>
>I have noticed upon comparing the protocol we received to others found on the internet that ours lacks the step in which the slides are placed in Bouin's fluid. There was also no specification in our protocol for the type of hematoxylin, so we had been using the same hematoxylin (Harris's modified with acetic acid) that we use in our H & E stainings. I was wondering if any of these might have led to the stainings not going as planned. The tissue sections also looked slightly brittle, perhaps due to a harsher stain. I am wondering if this has anything to do with my fixation process in which I use a 4% paraformaldehyde/PBS mixture, ethanol and xylene steps, as well as embedding the tissues in paraffin.
>
>Is Bouin's a crucial step, would I have better staining with a different tissue fixation, or should I be looking in a different direction? Any suggestions would be very much appreciated!
>Thanks!
>
>Amber Greenbank
>
>Arizona State University
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 24
Date: Fri, 06 Aug 2004 15:08:47 +0200
From: Martin Hasselblatt <hasselblatt <@t> uni-muenster.de>
Subject: [Histonet] Cytochrome oxidase (COX) staining:
non-carcinogenic alternative to DAB?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <411382DF.4030602 <@t> uni-muenster.de>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Dear Histonet members,
staining of snap frozen muscle tissue for cytochrome oxidase (COX) using
diaminobenzidine (DAB) works nicely in our laboratory.
We would be happy to know, however, if anybody is aware of an
non-carcinogenic alternative method to visualize cytochrome oxidase
activity.
Thank you very much for your help
Martin Hasselblatt
Martin Hasselblatt
Institute of Neuropathology
University Hospital
Münster, Germany
http://www.klinikum.uni-muenster.de/institute/npatho/mitarbeiter/hasselblatt/index.html
------------------------------
Message: 25
Date: Fri, 6 Aug 2004 09:15:39 -0400
From: "Hermina Borgerink" <hborgeri <@t> wfubmc.edu>
Subject: [Histonet] Masson Trichrome
To: <histonet <@t> lists.utsouthwestern.edu>, <Bdeer24 <@t> aol.com>
Message-ID:
<9AEEF1FB6254224AA355ED285F8491650999C853 <@t> EXCHVS2.medctr.ad.wfubmc.edu>
Content-Type: text/plain; charset="US-ASCII"
Amber,
Below is a modified version of the original Masson's Trichrome which I
have successfully used for over 40 years. Instead of using
Woodstain-Scarlet, which I don't think is available anymore, substitute
Crocein Scarlet which Sigma sells.
Masson's Trichrome (Modified)
Post-fix in Bouin's fixative overnight if primary fixation was not done
using Bouin's
1. Bring sections to water and wash for 5 - 10 minutes.
2. Stain nuclei for 15 minutes in Weigert's iron hematoxylin
3. Wash for 5 - 10 minutes in running water and rinse in 1% acetic acid.
4. Place slides in Woodstain Scarlet-Acid Fuchsin mixture for 5 minutes.
5. Rinse briefly in 1% acetic acid and differentiate in 5%
phosphomolybdic acid. Differentiate to the point where collagen and
ground substances are pale pink to colorless, usually about 30 seconds
to 2 minutes. 6. Rinse in 1% acetic acid. 7. Place for 15 - 20 seconds
in the Aniline Blue solution (Aniline Blue - Woodstain Scarlet - Acid
Fuchsin mixture). 8. Place for a quick rinse in 1% acetic acid. 9.
Dehydrate in four changes of alcohol. 10. Clear in xylene and mount in
permount.
Preparation of Staining Solutions:
Weigert's Hematoxylin:
Solution A:
29% ferric chloride - 4 ml
Distilled water - 95 ml
Conc. HCl - 1 ml
Solution B:
Hematoxylin - 1 gm
95% ethanol - 100 ml
For use, mix equal parts of solutions A and B. Use fresh each time
Woodstain Scarlet-Acid Fuchsin: prepare a 1% solution of Woodstain
Scarlet NS and a 1% solution of Acid Fuchsin (C.I. no 692). Mix 8 parts
of Woodstain Scarlet solution with 2 parts of Acid Fuchsin solution and
add 1ml acetic acid to 99ml of the mixed dye solution.
Aniline blue: To 85ml of 1% acetic acid add 5ml of a saturated solution
of Aniline Blue (C.I. no 707) and 10ml of the above Woodstain
Scarlet-Acid Fuchsin mixture.
Hermina M. Borgerink, BA, HTL(ASCP)QIHC
Wake Forest University Health Sciences
Department of Pathology
Medical Center Blvd.
Winston-Salem, NC 27157
Tel. (336) 716-1538
Fax (336) 716-1515
e-mail hborgeri <@t> wfubmc.edu
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End of Histonet Digest, Vol 9, Issue 8
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