[Histonet] electrical charge slides

[Bill Sinai]

Gayle,

I have no idea what the substance they use.  I was told by Menzel in Germany
that the process is a secret.  When I enquired about the "New"  Superfrost
Plus Ultra they informed me it was a different process than the Superfrost
Plus.

I wish I knew more.

The Superfrost Plus Ultra have to date eliminated the staining problems with
the Ventana Benchmark.

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Gayle
Callis
Sent: Saturday, 07 August 2004 2:12 AM
To: Bill Sinai; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] electrical charge slides


Bill,

Would you explain what is used for coating that contains calcium?  Does
Silane contain calcium?  Poly L lysine?  If there another coating we are
not aware of, the one containing calcium?  I have never heard this theory
before and am fascinated by your comments.

- At 08:54 PM 8/5/2004, you wrote:

>Dana,
>
>There was heaps written about this on Histonet previously, look in the
>archives.
>We found that the problem was associated with the Plus slides when used in
>conjunction with an EDTA buffer as the material used to coat the slides is
a
>calcium salt and the EDTA was affecting the coating by chelating the
>calcium!!!
>
>All the best
>Bill Sinai
>Laboratory Manager
>Tissue Pathology, ICPMR
>Westmead NSW 2145
>Australia
>Ph 02 9845 7774
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
>DDittus787 <@t> aol.com
>Sent: Friday, 06 August 2004 12:10 AM
>To: histonet <@t> pathology.swmed.edu
>Subject: [Histonet] electrical charge slides
>
>
>To my colleagues:
>
>I have a question.Is anyone out there having occasional staining(orlack of
>staining on IHc slides?) I was told that the charge or the way the slides
>are charged can have (pardon the pun) a negative effect. Does anyone know
>/experience or have the scientific data for this?
>                                  Dana
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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__________________________________________________________________

This electronic message and any attachments may be confidential.  If you
are not the intended recipient of this message would you please delete the
message and any attachments and advise the sender. Western Sydney
Area Health Services (WSAHS) uses virus scanning software but excludes 
any liability for viruses contained in any email or attachment.

This email may contain privileged and confidential information intended
only for the use of the addressees named above. If you are not the
intended recipient of this email, you are hereby notified that any use,
dissemination, distribution, or reproduction of this email is prohibited. If
you have received this email in error, please notify WSAHS
immediately. 

Any views expressed in this email are those of the individual sender 
except where the sender expressly and with authority states them 
to be the views of WSAHS.